We have had this issue previously.
We tracked it down to the brain biopsies arriving in "Isotonic" saline (which
is really not isotonic).
See: Henwood, A., (2007) “Adverse effect of saline on brain intraoperative
(frozen section) Histology” J Histotechnol 30(3):193.
Ask the surgeons to send
Hi Dorianne,
You need to freeze your tissue faster. Ideally, isopentane placed in a
metal cup, that is that is then frozen in a dewar of liquid nitrogen, works
best. The isopentane, once frozen, is thawed a little with a metal rod to
produce a small liquid pool and your tissue is
First of all, freezing spray should NEVER be used in a cryostat. It produces
dangerous aerosolized pathogens that linger in the air, just waiting to infect
someone. There should be no exceptions.
As to the ice artifact problem, not surprising to see this in brain since it
is so fragile and
Yes, definitely ice crystal holes! If the tissues are unfixed you will have to
freeze much more rapidly (isopentane cooled with liquid nitrogen.) If fixed in
formaldehyde, cryoprotect by immersing the pieces in 20% sucrose, until they
sink.
John Kiernan
Anatomy & Cell Biology, UWO
London,
Dorianne Bonello, Allied Health Practitioner (MLS), Histology Laboratory -
Pathology Health-Mater Dei Hospital, on the island of Malta asks:
> >>We are experiencing freezing artifacts on our frozen sections.
> Basically, we are seeing cavity-like structures under the microscope,
> mostly
Dear all,
We are experiencing freezing artifacts on our frozen sections. Basically, we
are seeing cavity-like structures under the microscope, mostly elongated,
especially when it's a frozen section on brain tissue. This is most probably
happening due to ice crystal formation. We're not using
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Message: 1
Date: Wed, 14 Jul 2021 22:03:24 + (UTC)
From: Cheryl
To: "histonet@lists.utsouthwestern.edu"
Subject: [Histonet] PathCentre (ThermoShandon) protocols - help?
Message-ID: <7393119.276682.1626300204...@mail.yahoo.com>
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Help??
