[Histonet] Improving wrinkles and bubbles in fixed-frozen mouse brain cryo-sections?

2021-08-09 Thread Anna Xiao Luo via Histonet
Dear List, I was cryo-sectioning fixed-frozen and fresh-frozen mouse brains, and found a lot of wrinkles and folding in the fixed-frozen sections, while the fresh-frozen sections were pretty smooth. Below are my sectioning conditions and observations. I would appreciate any advice on improving

[Histonet] combining cytology and histology

2021-08-09 Thread Richardson, Pam K via Histonet
Does anyone have a lab that has combined histology and cytology? I would like to know how they are staffed and if accessioning handles all samples or if they are handled separate? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical

Re: [Histonet] Histonet Digest, Vol 213, Issue 6

2021-08-09 Thread Wooten, Jennifer via Histonet
We just recently purchased the Epredia NX50, which we use with our older HM525s. We love it, I just wish they would make height adjustability a standard option on them! Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University Hospital

[Histonet] Wrinkles and bubbles in fixed-frozen mouse brain cryo-sections.

2021-08-09 Thread Anna Xiao Luo via Histonet
Dear List, I was cryo-sectioning fixed-frozen and fresh-frozen mouse brains, and found a lot of wrinkles and folding in the fixed-frozen sections, while the fresh-frozen sections were pretty smooth. Below are my sectioning conditions and observations. I would appreciate any advice on improving

Re: [Histonet] cryostat

2021-08-09 Thread Haiyan via Histonet
Hi Mehndi, Same here, I had been using Epredia cryostat for the last several years. I like it very much. A video on youtube shows its beautiful features. https://youtu.be/oMNt6RT5cWI Best, Haiyan On Mon, Aug 9, 2021 at 7:04 AM Morken, Timothy via Histonet < histonet@lists.utsouthwestern.edu>

Re: [Histonet] cryostat

2021-08-09 Thread Morken, Timothy via Histonet
Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two Epredia NX70 cryostats. We've used them for 8 years now and really like them. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

[Histonet] cryostat

2021-08-09 Thread Histology via Histonet
Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Leica anymore. Thinking of trying a different company maybe Tanner or Rankin. What does everyone use and like. We do not cut Mohs, only DIFs. Maybe 1 a day. Thanks