[Histonet] Vitality versus viability

2022-03-10 Thread Alida Bailleul via Histonet
Dear Histonet, What is the difference between cell vitality or cell viability? Is there a difference between a viable cell and a vital cell? Than you very much for your help All the best Alida Bailleul -- Dr. Alida M. Bailleul Associate Professor Institute of Vertebrate Paleontology and

Re: [Histonet] best paraffin

2022-03-10 Thread Bacon, Charles via Histonet
Over all of my testing I found there are several factors to consider when choosing paraffin: 1. Processor type 2. Tissue type 3. Use - embedding vs. processing The processor type my dictate limitations on paraffin selection. For example we were told not to use Paraplast Plus in a processor

Re: [Histonet] best paraffin

2022-03-10 Thread John Garratt via Histonet
Interesting that you are mixing the two. Is that common practice out there? John Sent from ProtonMail for iOS On Thu, Mar 10, 2022 at 9:40 AM, Cooper, Brian wrote: > Paraplast for processing and Paraplast Xtra for microtomy at our institution. > > Thanks, > > Brian D. Cooper, HT (ASCP)CM |

Re: [Histonet] best paraffin

2022-03-10 Thread Cooper, Brian via Histonet
Paraplast for processing and Paraplast Xtra for microtomy at our institution. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu

Re: [Histonet] best paraffin

2022-03-10 Thread Jay Lundgren via Histonet
Paraplast plus is my personal favorite. On March 10, 2022, at 11:04 AM, John Garratt via Histonet wrote: The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal

Re: [Histonet] best paraffin

2022-03-10 Thread John Garratt via Histonet
The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal preference. If you are having problems cutting it may well be related to other factors like fixation and

[Histonet] best paraffin

2022-03-10 Thread Rinker,Jeffrey via Histonet
I was wondering what people think is the best paraffin to use. I find it hard to get a good read on what is best for our lab. We do around 60-100 blocks a day and do a bx and surgical run overnight. I think that we are having problems with cutting because we are not optimized and i am trying