Re: [Histonet] Histonet Digest, Vol 232, Issue 11

2023-03-21 Thread Izak Dimenstein via Histonet
>From my Grossing Technology book: Gouty tissue contains monosodium urate crystals. Tissue specimens with gout diagnosis must be submitted in either Carnoy’s fixative (chloroform, acetic acid, and absolute ethanol, now almost abandoned in regular practice) or in absolute ethanol. Fixation

[Histonet] Tracking of blocks onto processors

2023-03-21 Thread Hagon, Christopher (Health) via Histonet
UNOFFICIAL Hi Histonetters, Just wondering if or how others are tracking/scanning racks of blocks onto processors. In this day and age of traceability with barcodes, we scan each block in each rack before loading onto a processor and recording which processor that rack went on to. Has anyone

Re: [Histonet] IHC staining of tendons and cartilage

2023-03-21 Thread John Kiernan via Histonet
You might like to look at this 1999 article from Microscopy Today, about keeping sections on slides. https://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan London, Canada. = = = From: Shirley A. Powell via Histonet Sent: March 21, 2023 2:56 PM To: Charles

Re: [Histonet] IHC staining of tendons and cartilage

2023-03-21 Thread Shirley A. Powell via Histonet
Hi Charles, Shirley Powell here in humid Georgia. I ran an IHC reference lab here for many years. I had a problem with using charged slides for a lot of the tissues I processed. I used manual and automation methods. My tissues were washing off a lot. I changed to an adhesive for the

[Histonet] IHC staining of tendons and cartilage

2023-03-21 Thread Charles Riley via Histonet
Hello all, I am in a new position and it will potentially require doing a lot of IHC testing on cartilage and tendon samples. I have done some practice runs on my automated stainer and manually and am running into issues with the tissue sections falling off completely or folding over on itself