Hi Esther,
I worked extensively with brain and spinal cord sections in the past, in
multiple species. You need endogenous quenching step.
I would always make my own (commerically available components yielded
different results). I used a 0.09% H2O2 in 6% Triton-X 100: (formula: 200mL
1x PBS + 6mL
Hi all,
Can someone please provide me with more details regarding cryosectioning of
mouse spleen and liver tissue?
Currently, I've been fixing my samples in 4% PFA (they are well fixed - I
know that will be the first question asked!), and then cryoprotect the
tissue in a series of graded sucrose
Good afternoon,
I am having a few issues getting nice morphology from both liver and heart
tissues. I currently work only with CNS tissues (brain, spinal cord, DRG,
etc.) but recently, our research team have become interested in
immunostaining some peripheral tissues, including the heart and
Hi Histonet!
I was wondering if anyone could provide me with a reliable H protocol for
fresh frozen tissue. Currently, we cut our slides on the cryostat and store
them at either -20 or -80 (depending on the study director) until they
require us to stain for morphology.
Typically the tissue isn't
