[Histonet] IHC positive controls

Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future

[Histonet] Ab vendors

Hello Histonet, Has anyone found vendors for Myc and LEF-1 Abs that are not RUOs? Thanks, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/

[Histonet] Test

Test Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!

To everyone who took the time to reply to my IHC survey, Big help!! Cindy Cindy Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Survey

section). 5) Use a bar code / tracking system? Thank you so much for your time on this matter, Cindy Cindy Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] Patti Loykasek - k/L staining

positive controls.Hope this helps, Cindy Bulmer HT(ASCP), QIHCIHC Supervisor, CTPLWaco, TX On Monday, June 1, 2015 3:43 PM, "Morken, Timothy" wrote: Patti, do remember any details of the following procedure" We have a heart pathologist  interested Thanks to you

Re: [Histonet] Dako CMV

I use the Dako CMV concentrated Ab with a 1:6000 titer using the Mouse LP and AR 6.0 Hope this help, good luck! Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From: "Stoll, Kathryn" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, S

[Histonet] HercepTest Kit K5207

Hello Histoland, Dako is experiencing a shortage of the FDA HercepTest kits, K5207, hopefully they will get more in the end of Sept. I am in panic mode, as I only have enough stock to stain patients through next week! Does anyone have enough stock to loan me a kit Our company will pay for

Re: [Histonet] Dako Hercept Kits

I'm using Dako Hercept Kit with lot number 20003874 and exp.date of 9-2014 and it is fine. Our previous kit was lot number 20003728, exp. date 8-2014 and it was fine. Hope this helps everyone. Cindy   Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX On Wednesday, May 7, 2014 4:33 PM

[Histonet] Procollagen 1

Hello Histonet, Is anyone using this Ab on paraffin embedded  human skin tissue that is not classified as an RUO? Thanks, Cindy   Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] control slides

Hello, Does anyone have a good source for buying HSV control slides for IHC? Thank you, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/l

[Histonet] Dako HercepTest Kit

Hello Histonet, Regarding the negative reagent control on the HercepTest Kit from Dako, order # K5207.  We wrote CAP about this and this was their reply: CAP would still require the laboratory to abide by any manufacturer instructions that may be more stringent than the CAP requirement. Since th

[Histonet] p53, control

What type of tissue is everyone using for a positive control for this Ab, and are you using Mouse or Rabbit Ab? Thanks, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://

[Histonet] IHC Stainers

Hello Techs,   I would like to get some feedback on the Dako Autostainer Link 48, using the Flex reagents.   Thank you, Cindy  Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://l

[Histonet] Positive controls

Hello Histoland,   Does anyone have a good source for purchasing HSV I & II ( Cocktail) positive controls?   Thanks, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists

[Histonet] HercepTest

I understand the HercepTest calls for a waterbath for the epitope retrieval process... but, I'm wanting to know what type of equipment, ie:  waterbath make and model or if people are using the Dako PT link module.   Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX

[Histonet] HercepTest

Hello Histoland,   Anyone using the HercepTest for the Dako autostainer, Code # K5207? If so, what equipment are you using for the Epitope retrieval process?   Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Hist

[Histonet] c-erbB2

Hello Histoland,   Has anyone seen any "B-9 ducts" staining positive with the c-erbB2 Oncoprotein (Rabbit, conc.) # A0485 from Dako?   Thank you, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.uts

Re: [Histonet] RE: DAKO Autostainer Link 48/FLEX system

Hey Loralee,   Could you post a few of the pros and cons?  Do you like the new system? Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX --- On Thu, 4/15/10, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system To: "Taylor, J

[Histonet] Job Opening

information.   Cindy   Cindy Bulmer HT(ASCP) QIHC IHC Supervisor, CTPL Waco, TX 254-752-9621 ext 447 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Job posting

Central Texas Pathology Laboratory in Waco, TX has a full time position available for an IHC technician.  Must be HT(ASCP) registered.  CTPL offers excellent benefits along with competitive salary.  Contact me for more information.   Cindy     Cynthia Bulmer, HT(ASCP)QIHC IHC Supervisor, CTPL Wac