Re: [Histonet] embedding of insects in historesin (Technovit 7100)

Hi Jurgen, If you still need assistance,please let me know. Feel free to contact me directly. Best, Damien L. On Fri, Nov 21, 2014 at 12:55 PM, dr.ha...@gmx.net wrote: Hallo, Andi G. Thanks for your reply. Normally I use paraffine for embedding insects after dehydration

Re: [Histonet] Peggy Wenk's passing

Very sorry to read this. Peggy was truly a wonderful person and will be missed. -Damien L. On Mon, Jul 28, 2014 at 10:57 AM, Colleen Forster cfors...@umn.edu wrote: So very sorry to hear of Peggy's passing. She was such as inspiration to us all. She will be greatly missed. May God grant her

Re: [Histonet] Clinical histology to Research histology

Hi Andi, Wow,I certainly hope that doesn't happen! I also hope you don't TOTALLY retire from histology. -Damien On Wed, Feb 5, 2014 at 10:46 AM, Grantham, Andrea L - (algranth) algra...@email.arizona.edu wrote: Agree, in spite of all the positives, the academic politics part is a big

Re: [Histonet] protocols to fix insect wings that are quite hirsute

Hi Jorge, I'd be happy to help you if I can. Please feel free to message me. Best, Damien L On Sun, Feb 2, 2014 at 10:45 AM, Jack Ratliff ratliffj...@hotmail.comwrote: This sounds like a question for the insect histology expert, Damien Laudier! He monitors this site so I am sure he

Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?

Hi Dorothy, I buy Methyl Methacrylate from Fisher Scientific. -Damien L. Sent from my iPhone On Jan 31, 2014, at 12:42 PM, Dorothy Hu abt...@gmail.com wrote: which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abt

Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?

Oh,that's awful! Fortunately,I've never had a problem with product from either company;comparable results. -Damien Sent from my iPhone On Jan 31, 2014, at 2:15 PM, Jack Ratliff ratliffj...@hotmail.com wrote: Dorothy, I personally order from Sigma and I have to tell you that in all my

[Histonet] Re: Processing Drosophila into paraffin

Hi Kathleen, Yes,95% ethanol is fine. It's (Methyl Benzoate) most likely misspelled in your protocol. Try Sigma catalog # M29903. -Damien L -- Damien Laudier Laudier Histology 917-836-7573 www.LaudierHistology.com * * ___ Histonet mailing list

Re: [Histonet] Re: undecalcified bone IHC

Excellent! Happy to read the venerable Neil Hand is coming back to the symposium, always a great speaker! -Damien On Tue, Mar 13, 2012 at 10:50 AM, Jack Ratliff ratliffj...@hotmail.comwrote: I might also add that Neil Hand is co-speaking with myself and Philip Seifert this year

Re: [Histonet] Question

. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Damien Laudier Laudier Histology www.LaudierHistology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Re:Goldner's Trichrome for Osteoid

pattern, or have the sharp contrast, that what would be observed when using a calcified bone section. For decalcified sections, I personally like the Gomori one step with Aniline Blue (osteoid will stain blue). 1.Yes (with some exceptions) 2. Formic acid works well 3. Try 5 microns -Damien L

[Histonet] Re: Scope of Practice

of wisdom ultimately proved to be true. Good Luck, Damien L. -- Damien Laudier Laudier Histology www.LaudierHistology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Removing MMA

tinctorial stain on your ground sections if etched well beforehand. -Damien L. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Bone ground sections

. For darker nuclear staining,I like Gill III or Ehrlich's. The Celestine Blue-Hematoxylin technique also works well on ground sections. -Damien L. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] Re: Deplasticizing before staining

___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re:Deplasticizing before staining

for more information on technique if you'd like. -Damien L. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Drosophila sectioning

Hi Gil, My histology service specializes in insects related invertebrates. I have a protocol that I can send you answer any additional questions you may have. Please feel free to contact me. Best, Damien Laudier ___ Histonet mailing list Histonet

[Histonet] Re: Insect/Arthropod histotechnology

Hi Ana, I work with insects and related organisms. What did you need to know? -Damien Laudier ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Cortical bone image analysis fluorescent calcein measurement (Damien Laudier)

adjustments. Once you fix this problem, yes it is possible on a 1mm section, but since all confocal microscopes are not created equally, success will ultimately depend on the resolving power of your system. Good luck! -Damien Laudier ___ Histonet mailing