[Histonet] Influenza A IHC

If anyone is still doing Influenza A IHC testing, could you share your vendor source and catalog number with me? I'm looking to find a better source than what I'm using right now. I don't do it very often (since we have a PCR test) but once in awhile it's requested, and my old antibody just

Re: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline

Hi Sandra, Being a mouse monoclonal, it may not cross-react with canines/felines; the epitope may be specific to human follicular cells only. The polyclonal Thyroglobulin from Dako/Agilent works fine on all the mammals I've tried it on so far (and a frog as well). Regards, Jan Jan Shivers

Re: [Histonet] Chromogranin A in dogs

Hi Lisa, I just worked up an antibody from abcam; cat. # ab85554 Rabbit polyclonal 1:1200 (0.6ug/ml) -1:1600 (0.4 ug/ml) Heat retrieval in pH 6.0 solution 45 min primary Ab, 45 min secondary Ab (polymer system), 15 min chromogen (AEC+) Regards, Jan Jan Shivers Senior Scientist IHC/Histology

Re: [Histonet] IHC Control Slide Longevity

Hi Sandy, We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same morning as staining; case material as well). We've never had any problems with Synaptophysin; it's always worked very well on unstained controls that are up to a year old (stored in the fridge). Best regards, Jan

Re: [Histonet] Not DAB IHC waste

Hello, It depends upon which 'red' chromogen you are using. There are several with different chemical compositions, so you will need to look at the Safety Data Sheets which pertain to the chemical you are using. In my lab we use 3-amino-9-ethylcarbazole (AEC) which is considered hazardous. It

[Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising

Re: [Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5

Hi Sandy, I get mine from CosmoBio, catalog # KAL-KT022. Needs heat retrieval and I go 1 hr incubation in primary Ab at room temp. Good luck, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333

[Histonet] Bordetella IHC

Are there any veterinary labs that do Bordetella IHC and can accept tissue from outside of the US? Asking for a colleague. Thanks in advance. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333

Re: [Histonet] Adenovirus antibody?

Hello Paula, This isn't an answer to your question, because I do use the Millipore antibody (MAB805) and it works quite well on a Dako platform or done manually. It requires enzyme digestion antigen retrieval. I use it at a 1:400 dilution (45 min) with a polymer detection system (45 min). I'm

[Histonet] EMA/MUC1 IHC on dogs

Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is your vendor? You can reply to me in a private message. Thanks in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota