[Histonet] PNL2 antibody

2022-10-24 Thread Jennifer Phinney via Histonet
Hello Histonet, I'm in a Veterinary Diagnostic Lab and I'm wondering if anyone has a supplier for a PNL2 antibody that actually works in species other an human. My lab typically uses the Millipore Sigma (Biocare) antibody, but it's been on back order since at least July and the available date

[Histonet] Sakura SmartWrite software issue

2022-03-16 Thread Jennifer Phinney via Histonet
Hello Histonetters, For those using the Sakura SmartWrite cassette printer, how many of you are using the SmartWrite Software V 3.1.0 that came out in 2019? We don't have the autoloader, so we print one cassette at a time. We recently updated from the 2017 version, and have ran into an issue

[Histonet] IHC Research Technician Kansas State Veterinary Diagnostic Lab

2021-06-22 Thread Jennifer Phinney via Histonet
Hello HistoNetters, The Kansas State Veterinary Diagnostic Laboratory (KSVDL) within the College of Veterinary Medicine is seeking applicants for a Research Assistant. This position will have the primary responsibility of coordinating new incoming research projects and developing new tests

[Histonet] Automated Coverslippers

2021-05-13 Thread Jennifer Phinney via Histonet
Hello Histonetters, My lab currently has a Leica CV5030 that is attached to our ST5010 Autostainer. We bought it way back in 2012 and it is starting to have progressively more issues working reliably. This is the only one that can be integrated with our stainer, so we know we'll have to get a

[Histonet] Crab histology

2020-10-30 Thread Jennifer Phinney via Histonet
Hello Histopeeps, Does anyone have experience with histology on crustaceans? My lab received a moon crab with shell lesions (of course) and I'm wondering what the best treatment is to ensure the lab can cut the best slides of the shell possible. I am currently thinking a combination of decal

[Histonet] Detecting copper in H staining?

2019-09-10 Thread Jennifer Phinney via Histonet
Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H slides. Is anyone aware of how this could have been achieved? I assume it would either

Re: [Histonet] Elephant Tissues

2018-09-06 Thread Jennifer Phinney via Histonet
any computer. On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet mailto:histonet@lists.utsouthwestern.edu>> wrote: Hello Histonetters, Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases re

[Histonet] Elephant Tissues

2018-09-05 Thread Jennifer Phinney via Histonet
Hello Histonetters, Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. Thanks for any help, Jennifer Phinney QIHC Kansas State University

[Histonet] Cut FFPE Slides for ISH

2017-08-28 Thread Jennifer Phinney via Histonet
Hello Histonet, I am hoping someone can point me in the right direction to find some references for how long FFPE slides intended for ISH can be kept before staining. I have a researcher who swears their slides have to be stained within 24 hours of being cut, which does not sound correct to