Re: [Histonet] Fast Green / Sirius Red - Unknown blue features

David We have found that mordanting in bouins (just like you would a Masson's trichrome) prior to completing a standard Picrosirius Red stain protocol that we get very consistent results. I think this method has been published but I do not have access to the reference currently. Its worth a

Re: [Histonet] Desperately seeking a certain IHC expert

Joe Myers biocate Sent from my Windows 10 device From: Maria Cruz via Histonet Sent: Monday, February 3, 2020 7:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Desperately seeking a certain IHC

Re: [Histonet] IHC on Pig Tissues

Lori You can't use a dual label detection system. We have found most mouse polymers will cross react with porcine tissue. We know that the dako one does. You need to use the rabbit envision for rabbit monoclonal antibodies or with mouse monoclonals you need to use a rabbit anti-mouse and

Re: [Histonet] H trouble shooting

That an image of incomplete dehydration the samples have not been processed properly and adequately dehydrated. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell

Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

You don’t need to heat up histogel in a microwave its quicker that way but if you set an oven at around 65 to 70 it will melt in around 20 minutes or so, that’s what we do. It can get messy in a microwave. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592

Re: [Histonet] Roche Symphony and Ultra users

It might be the coverglass we have seen bubbles in the glass itself, it looks like there is water on the slide, but it's actually bubbles in the coverglass Sent from my Windows 10 device From: Victoria Baker via Histonet Sent: Friday, August 30, 2019

Re: [Histonet] Antibody Validation CLIA

Keep in mind like Lacy said its cases and not slides so you could place multiple cases on one slide or create a simple array using disposable biopsy punches. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060

Re: [Histonet] Image analysis on breast markers

Teresa CAP should be releasing a paper soon, within the next couple months. Quantitative Image Analysis (QIA) for HER2 Immunohistochemistry for Breast Cancer, it will address the question that you have. Keep any eye out for it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier

Re: [Histonet] Mouse bladder

I would try histogel instead of agar. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com

Re: [Histonet] Beaker with or without Vantage

I'm a member of API - Association for Pathology Informatics and they have a list server similar to the histonet and there is always posts about Epic Beaker. You might find some information on their website. https://www.pathologyinformatics.org/ Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Re: [Histonet] Tracking lot numbers

Teri When we change the tissue processor or H stainer instead of just recording a "check mark" that the solution has been changed we record the lot numbers on the form instead. We also have a from that is posted above the tissue processor that we fill in with dry erase the current lot numbers

Re: [Histonet] Old control blocks

Elaine We have the temperature of our cryostat calibrated yearly by an outside calibration company. If the thermometer you are using inside the cryostat has been calibrated and is traceable I think you might have an issue with the temperature reading that is being displayed on your cryostat.