ProtK digestion at 55 degrees C for half an hour will do fine
There is also a new FISH like technique to detect single RNA molecules in situ,
can't remember the name atm. But it works with so called Z-probes and it's very
specific and sensitive. Especially when you want to look at virus in tissu
Check out www.cqpath.com, nice references from a2z ;-)
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Op 16 mrt. 2013 om 18:05 heeft "histonet-requ...@lists.utsouthwestern.edu"
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Check www.cqpath.com for more info
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Op 7 dec. 2012 om 19:03 heeft "histonet-requ...@lists.utsouthwestern.edu"
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Hi Histonetters,
I need some prostate experts to evaluate some virtual slides for a world wide
morphology ring trial. We started the ring trial already in August, but a few
of the participants don't respond. If you know anyone who would be interested,
please ask them to contact me (m.ka...@eras
Dear European histonetters,
Please follow this link for more info: http://www.no-cuts-on-research.eu/
Sign the petition and share the link with your peers!
Thanx,
Marcel Kap
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Hi there,
Just take dog's appendix or lymphnode and titrate the antibody until it looks
like the reference on www.cqpath.com
good luck!
Marcel
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of
his
Hi histonetters,
We formed an alliance with our virology department. They use virus cell culture
to determine virus titers and such, so I came up with the idea to let them
culture loads of cells, infect them with all kinds of viruses and than simply
make paraffin blocks of the cells.
They use
Dear Histonetters,
Some of you visited the Facebook page to find some nice pictures instead of the
CQPath site, which was disappointingly down. After a server crash this weekend,
we are back online! Once again I want to kindly invite you to take a look at
our IHC QC/QA/QI program.
Please take
Dear all,
It is finally there! A web based application to do all tedious IHC QC/QA/QI
related analysis for you. Completely inline with ISO 15189 and LEAN/Sigma6.
Please check our Facebook page and like anything you like. Where is the "I LOVE
IT!!!" button when you need it?!
Have fun at https:/
Dear all,
On behalf of the SPIDIA consortium (www.spidia.eu), I would like to
invite urology/prostate pathologists in your network to
participate in the SPIDIA Prostate Tumor Ring Trial.
The ring trial consists of the evaluation of 15 digital H&E slides, this
will take approximately 20 minutes o
check .cqpath.com : all you wanted to know about IHC but were afraid
to ask ;-)
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Dear all,
We have developed CQPath for our QC/QA. Still under construction at
www.cqpath.nl (of course also in English). Hopefully the TQM module will
be available soon.
Best regards,
Marcel
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@15
Wrap tissue in moist, not too wet!!, (0.9% salt or PBS) gauze and store
at 4 degrees C. No/hardly any harm done to morphology, antigenicity, RNA,
DNA and so on. Tissue is tougher than you think...
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027704
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