[Histonet] IHC on Bones

2011-12-30 Thread McCabe, Sara J.
Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended

[Histonet] PMS2 Mismatch Repair

2011-10-28 Thread McCabe, Sara J.
Hello Histonetters, Wondering if anyone is using PMS2 Mismatch Repair Enzyme from Novocastra/Leica. Have tried varied dilutions and retrievals without any luck. Any suggestions would be great! Thanks! Sara McCabe, BS, HT (ASCP) DuBois Regional Medical Center This email and any attached files

[Histonet] CMV IHC

2011-08-26 Thread McCabe, Sara J.
Happy Friday Histonetters! Anyone performing CMV by IHC? If so what are you using for a protocol? We ordered CMV from Cell Marque but they give no suggestions for a protocol. We are using the Leica Bond for our IHCs. Any input is greatly appreciated! Thanks! Sara McCabe, HT (ASCP) DuBois

[Histonet] RE: controls on patient slide

2011-08-12 Thread McCabe, Sara J.
We always put our patient and control tissue on the same slide. We have many controls already cut and then we place the patient tissue below. If we are using blanks that were previously cut then we cut the control and place that below the patient tissue. We chose to not invest in the

[Histonet] RE: p16 on the Bond

2011-08-12 Thread McCabe, Sara J.
Hi Nancy, We too use p16 on the Bond Max and Bond III using Leica ER 1 for 20 min. It is working great for us! Sara McCabe, HT (ASCP) DuBois Regional Medical Center From: histonet-boun...@lists.utsouthwestern.edu