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Op vr 22 jan. 2021 9:53 p.m. schreef Hans B Snyder via Histonet <
histonet@lists.utsouthwestern.edu>:
> Hello Cynthia,
>
> We have HP blocks. Our tissues are great for IHC but not special stains.
> Please contact me directly if you are interested.
>
> Thank you
>
> Hans B Snyder
>
Hello everyone. I was wondering how your thoughts are about automating your
lab (aka auto embedders and microtomes that are like a robots) what are
your experiences?
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I am also interested in that i run a small lab and would love to go xylene
free!
2017-02-23 12:21 GMT-06:00 Blake Taylor via Histonet <
histonet@lists.utsouthwestern.edu>:
> Our hospital has asked us to investigate going xylene free. Has anyone
> recently made a switch to a xylene alternative
Hello Everyone,
I have a co worker who is wondering if it is possible to use mouse or rat
antibodies on chicken tissue? Could someone help him with this, i have no
idea how to help him
Thank you
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Good day everyone,
I was wondering if anyone has any experience with black gold, for staining
myelin in brain sections, and is willing to share this protocol.
Thank you
Maarten
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I am also very interested in this! please let me know a good adhesive for
IHC
2016-09-12 9:05 GMT-05:00 Jessica Riggleman via Histonet <
histonet@lists.utsouthwestern.edu>:
> Hello,
>
> We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.
>
> We have had issues staining H
THank you all for the quick and wonderfull responses! I have some things to
work out now and i will let you all know the result
Thank you all so much
Maarten
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Hello everyone,
I was wondering if you could help me out. I have some chicken eyes for
research and i need a good fixation. My problem is that if i fixated the
eyes in formalin for 48 hours, and i cut open the eyes, the eyes shrink a
lot.
Do i need a different fixative? or do i need longer
Dear Rebecca,
Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.
Good luck,
Maarten
2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet@lists.utsouthwestern.edu>:
> I
Dear histonetters,
Since a few months, i started working in a histology lab, run only by me (
coworkers are not specialized in histology). There has not worked here a
person at histology for about 2 years.
After many new protocols, i decided to clear out some chemicals.
Now i found around 1 KG
Hello Everyone,
I am completely new to the technique, i tried several different protocols
but it doesn't seemed to succeed that very well. Does anyone have a
protocol for the Golgi Kopsch, where i have to include the tissue in the
end with paraffin?
Thank you all for your time
Mwerdler
UNAM
What i would do, melt the blocks you made.
1. Put them back in paraffin for one hour
2. Put them back in another paraffin or one hour
3. Put them in xylene for one hour
4. Put them in another xylene for one hour
5. Put them in another xylene for one hour
6. Put them back in paraffin for one hour
Hey Charles,
Why are the first 2 steps of formalin that long? If you make sure your
tissue is well fixated, then there is no need for such long fixation in the
machine. You could use step 2 for just washing the formalin out, so for
only like 5 minutes. You could use that time, to add to other
Good day everyone,
Does anyone have expierience with the vibratome OTS 5000? We just got this
machine and we have no idea how to work with it.
Thank you
Maarten Werdler
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