[Histonet] preordering giemsa stains

Hello, Is it a common practice to preorder stains that involve the stomach searching for H pylori? Or, is it more common to order as needed? Thank you for anyone's feedback. Paula Lucas Lab Manager ___ Histonet mailing list

[Histonet] Marking Pen

Hello, We have been using KP Marker Plus pens for cassettes. We have 2 Leica Processors and 1 VIP6 processor. The cassettes that go into the VIP6 are smeared and some are almost smeared off completely. The processors have the same solutions in them. If anyone can shed some light as to

[Histonet] Expiration date after opeing a reagent

Hello, We just got CLIA inspected and had no deficiencies. The inspector did say he wants to see us write down on any reagent containers when it was opened, and write down the new expiration date. The containers have the expiration date on them from the manufacturer, but the inspector said

[Histonet] tissue in alcohol too long now brittle

Hello, This is a question for a colleague of mine. Her routine tissue samples were left in 95% alcohol for 2 hours before she had a chance to get to it. She finished the processing, but now the gi's are extremely brittle and have holes. She is afraid of reprocessing and doesn't know what to do.

[Histonet] Leica Helicobacter pylori

Hello, good morning Has anyone tried this antibody (7 ml predilute) on your system? We have the Bond III and I was hoping to gain some information on where to start the staining parameters for this antibody. Thank you, Paula ___ Histonet

[Histonet] black staining in IHC

Hello everyone, We are running some markers on an Ascites fluid specimen, and there are grainy black pigments in the same area in the cell block for each marker. The markers we are running are CA125, ER, Mammaglobin and P53. Can anyone tell me what this could be? Thank you, Paula

[Histonet] cd138

Hello Histonetters, We have a Bond III and use the Refine Detection Kit for our antibodies. We've had no issues with this mentioned antibody but recently, we have seen background staining and have used 2 different lots. Both the plasma cells AND stromal cells are stained in endometrial

[Histonet] IHC background

Hello everyone, We have the Bond III and we ran an S100. We placed 2 serial sections vertically on the slide with the control tissue at the top for staining. The patient's section at the top underneath the control tissue had background staining, while the section on the bottom of that had no

Re: [Histonet] Leica Prisma problem

via Histonet Sent: Thursday, September 22, 2022 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Prisma problem *** This is an External Email *** Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed

[Histonet] Leica Prisma problem

Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas

[Histonet] microtome pm

Hi everyone, How often do you PM your microtomes? Currently, we have them done every 6 months but I'm thinking once a year is sufficient and I wanted to get some input before we decide. Thank you in advance, Paula Bio-Path Medical Group ___

[Histonet] Formalin Neutralizer

Hello, Can you recommend the easiest and most cost-effective product to neutralize the formalin waste from our tissue processors? Thank you, Paula Lucas Bio-Path Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] TB/mycobacterium for iHC

Hello, Currently, we send out for TB/mycobacterium for IHC and would maybe like to do the stain in-house if we could. We have the Leica Bond. Can anyone recommend a vendor for us to try that will work? Thank you in advance, Paula ___ Histonet

Re: [Histonet] "cooked" biopsy

Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ____ From: Paula via Histonet Sent: Thursday, 8 July 2021 04:53 To: 'Erick Rodriguez' Cc: histonet@lists.utsouthwestern.e

Re: [Histonet] "cooked" biopsy

ter boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet > wrote: > > Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrun

[Histonet] "cooked" biopsy

Hello, good day, Our pathologist is complaining about the tissues today that they are "cooked, burnt, crushed, shrunken" those are the adjectives she is using. Can you tell me the cause? Usually, the work comes out beautiful but today they are not. Nothing has changed on our processing

[Histonet] Paraffin Preference

Hi everyone..good morning. I have samples of Fisher Scientific..or maybe it's Richard-Allan's paraffin. It's called Histoplast PE. We use Leica's Paraplast Plus currently and I would like to know if anyone can share their preference and if you can offer any comments about the differences

[Histonet] Leica Bond, ASP6025 Processor parts

Hi all, Does anyone know of a Biomed company that makes and/or sells parts for the Bond III and the ASP6025 processor? It's amazing how much Leica wants for their parts. For example, the waste bottle used to collect the buffer waste is $674 and the seal for the retort's lid is $597.

[Histonet] IHC validations

Hello, I see 10 positive and 10 negative cases for CAP guidelines, but what about for CLIA? What is their guideline to validate an IHC antibody? Thanks in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] UV Light in Fume Hood

Hello everyone.. I hope all is well. My admin wants me to look into possibly adding a UV light inside our fume hood, which currently does not have one. I'm thinking maybe it's not as effective to kill viruses as we think it would be. If anyone can share your thoughts about it, or if

[Histonet] Diastase

Hello, We've been using STATLabs Diastase for our PAS with diastase digestion but they have a backorder until January. Can anyone recommend an alternative from other vendors? Thank you in advance, Paula ___ Histonet mailing list

[Histonet] UV LIGHT BOX to kill BLOODBORNE PATH

Hello, Has anyone ventured out and looked into a UV light instrument, like a box that can kill any bloodborne pathogens that are on paper (grossing area..where the patient req form is used)? The administrator has a grand idea about placing any paperwork that contains smudges/smears inside

[Histonet] contaminating patient requesition forms

Hello..good morning, Can anyone recommend a creative approach to avoid blood from being transferred from disposable gloves onto the patient requisition form during the grossing phase of histology? I've seen a number of smudge marks and have asked the grossing doctor to avoid touching the

[Histonet] Chromogenic ACD RNA ISH probes

Hello everyone, We recently received informative handouts regarding this technology from our Leica rep and we are in the process of scheduling a meeting to learn more about it. They say it's so wonderful, our jaws will drop from how beautiful the stains are. Can anyone comment on this

[Histonet] Hologic T2000 error code

Hello and good day, Can anyone tell me what the error code 76 is? We have 04 as well, and that means the "filter already wet". It comes up right after starting a run so I'm thinking it could be vacuum or waste line issue. Thanks in advance, Paula

[Histonet] Frozen Gel Pack with paraffin block for shipping?

Hello, This question has come up here at work a few times. Apparently, the department does different protocols, depending on who is doing the job of shipping out a paraffin block for additional testing. Our transcription department handles this and I was asked to look into it so I can submit a

[Histonet] sakura prisma stainer problem

Hello..Tech support with Sakura is not open yet so I was hoping maybe someone can help me here. We have words on the computer screen for the Prisma stainer that shows something that we haven't seen before. The words, "Maintenance Program" is there. We shut it down and turned it on to get to

[Histonet] Instrument Correlation

Hello everyone, Could you recommend/suggest how the correlation between 2 IHC Bond instruments is performed and written out for them? This has never been brought up before and I'm just starting my research. Thank you in advance, Paula ___ Histonet

[Histonet] Pathologist Assistant- Part-time

Hello, We are small private laboratory in Fountain Valley, California and we are looking for a licensed PA. If interested and if you have any questions, please contact Jason Cochran, Russel Blayney or Paula Lucas at 714-433-1330, or simply respond to this email. Thank you, Paula Lucas

[Histonet] Disposing Paraffin

Hello all.this is an on-going topic with our lab. If anyone can comment about your experience with paraffin waste, I appreciate that in advance. Does your lab or state requirements classify paraffin waste as "hazardous, bio-hazardous, RCRA or other"? Do you dispose as part of Red Bag

[Histonet] IHC and Histogel

Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Antibody Validation CLIA

Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20

[Histonet] holes in GI tissue

Hello, Our pathologist is complaining about "holes" in the GI tissue. This is what he mainly reads, so it could be other types of tissues and the other pathologists don't complain about it. Anyway, he showed me an example, and I see what he is talking about. I think it comes down to microtomy,

[Histonet] Glycophorin

Hello all, We have the Leica Bond and I am trying to get the Glycophorin antibody to work on our Bone Marrow Core bx's. Can anyone share the protocol for this antibody? I've tried Enzyme 1, ER 1 for 20 minutes so far. I'm thinking it could be the decal that is interfering but I would like to

[Histonet] Grosser Needed in Southern California

Hello, We need a true PA (Pathologist Assistant) or a highly qualified Grossing person that can do hospital work. Full time or Part Time options. If interested, please contact Jason Cochran (Administrator) for more information. Bio-Path Medical Group Fountain Valley, CA 92708 714-433-1330

[Histonet] VIPE300

Hello, We're looking at purchasing a refurbished VIP E300 and I was hoping for some feedback on this processor. Is it reliable, dependable, good workmanship kind of machine or are they nothing but trouble? Thanks for any replies and have a good day. Paula

[Histonet] P16

Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab

[Histonet] Wrist pain from embedding

Hello, Does anyone have a remedy for wrist pain while embedding or can offer a suggestion on what to wear during the embedding process for one of my technicians to wear? She'll be off the bench for a little while but I was wondering if there is something that would help her. Thank you in

[Histonet] BD Surepath VS Holgic Thinprep for Non-gyn speciemens

Hello, We're looking for a lab in the Orange County, California area that is preparing non-gyn samples using the Surepath system. We would like to submit a sample to evaluate the BD Prep stain slide processor for non-gyn specimens. We would like to compare this to the Hologic's Thinprep

[Histonet] Transcriptiionist needed Orange County, California

Hello, A very experienced laboratory who has been in the pathology business for over 30 years is looking for a transcriptionist to join our qualified staff. If interested, please contact: Russell Blayney 714-433-1330 phone 714-755-2984 fax rblay...@biopath.org Thank you, Paula Lucas,

[Histonet] Job Opportunities/Orange County, California

Hello, If you or if you know someone who might be interested in the following jobs, please inquire to Russell Blayney at 714-433-1330: 1. Transcriptionist- Full- time 2. Medical Biller- Full-time 3. Laboratory Courier- Part-time morning hours Thank you, Paula Lucas

[Histonet] Per Diem Histotech

Looking for a per diem histotech that can embed and cut our cases during requested vacations throughout the year. We start our day between 430am-7am, so any time to start in this time period works for us. Please send resume to plu...@biopath.org or fax to 714-755-2984 Thank you, Paula Fountain