Our S100 is not working very well. I think it's too old. Can someone
recommend an S100 antibody that works well in dog? It would be really nice to
find a good monoclonal instead of the polyclonals.
We are looking for schwannomas, astrogliomas, malignant melanomas and their
metastases.
I am looking for a less expensive antibody diluent and would like some opinions
on which companies are best . Are there differences between diluents when it
comes to background staining?
Margaret Perry HT(ASCP)
Veterinary Biomedical Sciences Department
North Campus Drive Box 2175
South Dakota
I would like to get your opinion about rabbit/mouse universal polymer detection
systems. Are there any out there that have little or no background when used
on canine ,feline, ovine, bovine, porcine, equine or caprine tissues? Right
now I use separate polymers for rabbit or mouse antibodies.
Is there another name for Nile Red? Could it be a florescent dye? I couldn't
find it on the Biological Stain commission or in a reference book from Harleco.
I don't know what the stain will be used for.
Margaret Perry HT(ASCP)
Veterinary Biomedical Sciences Department
North Campus Drive Box
Where do you buy the Sempermed gloves?
Margaret Perry HT(ASCP)
Veterinary Biomedical Sciences Department
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
I found something at tedpella.com. It's a glass bottom dish, catalog number
14026. There are several sizes. It looks similar to the one you posted.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
We are in need of some help trouble shooting this stain. Is it normal for the
tissue nuclei to stain red? If not what are we doing wrong? The Chlamydia are
staining OK.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD
Has anyone used this antibody on dog? If so which company did you use? Would
you be willing to share your protocol?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
I am curious how big an explosion there would be from 1% picric acid in acetone
if a little dried around the cap.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
We are in the market for a new HE stainer. We probably won't be doing any
special stains with it. Does anyone use the Shur/stain automated slide
stainer by TBS? If so what kind of repair record does it have? Are you
satisfied with it? Thanks for any info you would like to share.
Margaret
We are running out of our circo antibody from Iowa and did not find it listed
in their bank. Are there any recommendations for a different source?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
I am trying to work up Laminin in dogs and cats. I have tried the antibody
from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR
with no success. I would like to hear from anyone who is testing for laminin.
Do I need to use a different antibody?
Margaret Perry
I am posting this again to see if anyone has worked with nonstructured
proteins. Do they form the same crosslinks when fixed with formalin? Would
antigen retrieval be of any use? Do they react with IHC the same as structured
proteins?
Margaret Perry HT(ASCP)
Dept of Veterinary and
Does anyone have an e-mail address for Dick Dapson, Clive Taylor, and William
Grizzle?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
___
Histonet mailing list
Have any of you worked with the unstructured proteins? Do they form the same
crosslinks when fixed with formalin? Would antigen retrieval be of any use?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
We have the same problem. All we do now is make up the solution and leave out
the Chloral Hydrate. I haven't talked to the pathologist about it so I don't
know if it worked OK.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
We want to use laminin and Collagen IV to sort out the origin of some spindle
cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly
show laminin and collagen IV basement membranes vs. Fibrosarcoma which does
not. We need antibodies that will work in both cat and dog.
Hi
I was given blocks today of a canine tooth that had been decaled for 8 days.
We processed it on our regular overnight program. It was too hard to cut and
popped out of the paraffin. The biopsy trim sheet did not indicate it was a
tooth so I put the pieces in ammonium hydroxide for 10
Has anyone used the LabPulse microwave processor from EBS? We need a new
microwave for staining and retrieval and thought maybe we could get one that
will do processing also. I've looked at the Shurwave from Fisher, and the
Mars from Hacker. Are there any other companies that have small
Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit
specific HRP and used Nova red from Vector or the DAB in the kit depending on
which antibody we used. It is excellent , maybe even a little better than
Dako's envision. I did have to use blocks such as FC
What antibody would you recommend using to label the liver endothelial cells?
Is factor 8 our best marker? Also do you know of a special stain other than
HE that would demonstrate these cells?
Thank you.
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota
Our lab would like to know how you handle the quality issue of verifying that
the control material you use for special stains is what it's supposed to be.
For example: how do you confirm that bile in a piece of liver used as a Halls
control is actually bile?
We use controls for bacteria or
From: Perry, Margaret
Sent: Monday, September 27, 2010 11:41 AM
To: 'tgo...@mt.gov'
Subject: WNV
We have a problem with the equine tissue as do other labs. A lot of samples
can be done and only one will come up positive. We have good luck with avian
tissues. We use DAKO envision
Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company
good to work with?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
___
Histonet
I am using aqueous mounting media, faramount, for the first time. The slides
I coverslipped several days ago look dry. Will soaking in water remove the
coverslip?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
Has anyone tried the new polyvalent polymer detection EXPOSE from AbCam?
What did you think of it?
Margaret
SDSU
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Do you put the slim jims in formalin and then process them or just put them in
the processor?
Margaret
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
We use formula 83 for everything and do not have a problem with a film. We
have found that we must use isopropal alcohol instead of reagent alcohol in the
final dehydration steps.
Margaret Perry
SDSU
___
Histonet mailing list
In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered
paraformaldyhyde for the 37% formalin or do I need to buffer it?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Now that I'm done with my rant I have a real question. We are trying to do a
gram stain on fish and the safranine O is staining everything red. What other
stain would you use? I usually have time to look at the books but
unfortunately it's almost quiting time and the slides need to be done
We sometimes have problems with the stain if we use positive slides.
Margaret
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Is there a list serve for elisa's?
Margaret Perry
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
We want to try the double stain alcian blue Van gieson. Do you stain for the
alcian blue first and then the Van gieson? We do both stains but I am unsure
of how to put them together.
Margaret Perry
South Dakota State University
___
Histonet mailing
Are there good mouse antibodies that will work on dog and cat for the following:
Factor 8
GFAP
Mycobacterium sp
S100a
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Do you know of anyone that sells Klotz fixative? We use it in a pre vet class
but would like to eliminate the choloralhydrate since it is such a hassel due
to it's being a controlled substance. Is there a substitute fixative that would
retain the properties of Klotz?
A researcher wants to measure the length of the intestinal crypts and asked for
a suggestion on what type of stain to use. I am thinking of using a
phloxine/tartrazine stain and do a double stain with a GMS to show the basement
membrane. What is your opinion on this?
Do you have other
We are trying to cut fish scales that have been decalcified. They are chunking
out when we try to cut them. I think we need to soften the keratin and I
looked in the archives for the right dilution of ammonium hydroxide to use.
One post said 5% the other said straight. What dilution do you
Hi JoAnne,
Our pathologist requested we try a Faulkner stain for Mycoplasma. I found two
methods. The Faulkner/Lillie and the Faulkner/Kerr variations of the Warthin
Starry. One of these is a lower acid. The pathologist is looking up the
reference and will call the people who wrote a paper
We are trying to work up this stain per pathologists special request. We
usually do the Warthin-Starry method not this modification. I have the
published article from Stain Technology Vol. 20 No. 3 July 1945. The protocol
is a little vague and I have some questions? Step one brings the
If we need to hold retrieved slides for awhile we hold them in water up to 4
hours. Holding in water overnight leads to no signal. We have also tried
holding the slides in our Tris buffer and have found decreased or no staining.
Margaret Perry
___
I am trying a protocol that uses 0.1M Tris/0.15M sodium chloride pH7.5, which
is what I normally use. At another step in the protocol it says to wash with
0.05M Tris buffer pH 7.5.
What do I use to make the 0.05M Tris buffer? Is it a combination of Tris HCl
and Tris base without sodium
We are in the market for a new embedder. What do you suggest? I tried
searching the archives but only came up with older comments.
Vendors please feel free to contact
frank@sdstate.edumailto:frank@sdstate.edu. We may have to go with a
used instrument so would like comments on good
Has anyone found a good antibody for west Nile FFPE that will work on horses?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
I would add an eye wash station and a chemical spill kit to the list.
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
Our processor has gone down but we can process up to the paraffin step. My
question is how long can we keep the tissues in the formula 83 without any
visible problems? We would like to start processing tonight and possibly
infiltrate by hand tomorrow. Not optimal but it does work. Your
I'm trying to find a source for protease XIV other than Sigma. Any suggestions?
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
Patsy I have a couple of questions for you but have the wrong address.
Margaret Perry
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Please help! We have been trying to cut whole eyes from a pig. They need to
be nice enough for a publication. We are having the devil of a time because no
matter what we do there are wrinkles. If we turn the waterbath up to stretch
things out the retina detaches. Do any of you have
A few years ago we also had this problem. The brains were terrible. We found
that changing our processing chemicals, especially the wax, more frequently got
rid of most of our problems. We use Fisher plus slides. There is a difference
in plus slides. I recommend you contact several
We are looking for a source that sells a lid that looks like wax covered
cardboard. We use them on top of the Shandon slide containers holding xylene
or formula 83 while waiting to coverslip. They are about 5x7 and very thin.
They have been here for years so I have no idea where they came
50 matches
Mail list logo
