WHO is going to discontinue HPV testing?
Dana Settembre
University Hospital -UMDNJ
Newark, NJ
USA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
barbara.cr...@lpnt.net
Sent: Tuesday, September 25, 2012
Hi Chris,
CAP actually has a specific checklist question on Negative Controls.
The question is ANP.22570 and if
I remember correctly it is quite extensive. Most of the CAP questions are
about 7 to 10 lines. The Negative control question is more than a page long.
Get your hands a copy of the most
Carl,
H202 mixed with Buffer here.(instead of aq.)
That's OK too, right?
Dana
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Tuesday, July 10, 2012 11:32 PM
To:
Best of Luck to Mr. Burchette.
Who, I am sure, doesn't know me from Eve, but once sent me a positive control
block.
I didn't ask for anything in return.
I was happy astonished.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-Original Message-
From:
Looking to get current pricing and availability from mtm labs on p16 and no one
answers their phone - 800# nor their direct#
Seems Roche has taken them over.
Need to reach them.
Any help would be appreciated
Dana Settembre
Immunohistochemistry
University Hospital - UMDNJ
Newark, NJ
USA
From: Amanda Kelley [mailto:akell...@slu.edu]
Sent: Tuesday, June 12, 2012 12:13 PM
To: Settembre, Dana
Cc: Vickroy, Jim; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] looking p16 - where is mtm labs?
You have to get current pricing on P16 from your local Ventana rep
You should pose that question to CAP via
acc...@cap.org
they answer quickly and when they do you can print out their answer and keep
for when your inspectors come.
When I asked them that question, they said that it was usually at the
discretion of the pathologist in charge. But instead of
We are saving slides from inspection to inspection, about 2 years.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Thursday, April
I would be worried too.
Wearing gloves in the lab is always a good practice and
I believe, a requirement in my lab whenever handling reagents or
the possibility of coming into contact with reagents.
You have the right to wear gloves if you want.
Dana Settembre
University Hospital - UMDNJ
Tom,
As Tunde says, the Checklist 7/11/2011 question ANP.22570 does explain that you
can do as your pathologists suggests,
BUT there are rules to be followed if that is what you will do.
A negative tissue control must be processed for each antibody in a given run.
Any of the following can
Hi Donna,
I use Dako's Myo-D1 cat. M3512. Use it at 1:25
I use Dako's labeled polymer kit, Envision + Mouse.
Use Trilogy (from Cell Marque) in a steamer. That means:
Start the steamer and put a two Coplin jars in filled with Trilogy.
Place all your slides in 1st Coplin jar for 30 min. right then,
Her-2 IHC
Dana Settembre
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Coppin, Margaret
Sent: Wednesday, August 31, 2011 5:53 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP slides
Leica sells the NovoCastra line pTEN. That's what I have been using.
Which vendor have you been using?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis
Sent: Monday, August 15, 2011 10:41
We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Tuesday, August
Hi Nancy,
I am using the p16 with the Bond.
I am using the Bond's ER1 for 20min with their Refine detection kit.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
The only company currently allowed to sell it (have it, etc.)
MTM Labs 1-866-686-5227
CAT#: 9581
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
This is so much fun.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting
Sent: Thursday, June 30, 2011 2:50 PM
To: histonet@lists.utsouthwestern.edu; McAuliffe, William
Subject: Re: [Histonet]
Want to purchase Pax-2 for FFPE human tissue.
I have been told that there is a countrywide issue
that has it on BACKORDER - I have been waiting for about a 3 to 4 weeks.
I have been told that a release date may come in a couple of months.
I. Does anyone know enough to confirm this?
II. Where do
Validate.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ 07103
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague
Sent: Thursday, June 16, 2011 10:34 AM
To:
Laurie, when we called CAP they also told us to use Code 11.
That's a GOOD point - specimen unsatisfactory is this issue.
Dana Settembre
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
ANTIBODY LOTS
I have a Dako and I have a Bond too.
For the Dako I use my positive control. I use 3 slides.
One is treated as the negative control - no antibody
One is treated with the current lot
One is treated with the new lot.
I keep all QC slides together.
I keep the 3 slides together.
For my
Hi All,
Having a tough time looking for a reliable vendor for Pax-2.
What vendors are you using?
I need to use this on formalin fixed paraffin embedded human tissue.
What control do you use?
Thank you.
Dana Settembre
Immunohistochemistry Lab
University Hospital - UMDNJ
Newark, NJ
Lab Week is April 24th through April 30th.
Dana Settembre
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yahoo
Sent: Monday, March 28, 2011 9:26 PM
To: Histonet
Subject: [Histonet] Lab week
Can someone
Yes, you should run negative controls with each run - today and tomorrow.
Something can happen in tomorrow's run so tomorrow's negative control
will have different issues from today's negative control.
The negative control should go through everything that your
slides go through, whether it's
Hi Linda,
I found Dako's CD21 mouse antibody reliable.
M0784
Dana Settembre
University Hospital - UMDNJ
150 Bergen Street
Newark, NJ 07103
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Has anyone had any problems with the Leica Bond Max's
Heater assembly. We have 2 Bonds that are less than
2 years old and one of the heater assembles went.
That is 10 less slides that we have to work with.
Thank you,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
973-972-5232
Hi Cindy,
That too has happened to me and the process that you do to remedy it,
works for me too. (take off coverslip properly, re-apply DAB, and you
get a reaction.) My experience is that the slides are not shot, as described
by another Histonetter.
There was definitely something else going on.
Hi Jean,
I use Leica's MBP which the get from NovoCastra cat. NCL-MBP
I use it on formalin fixed paraffin embedded human tissue on my Dako.
I use Dako's Target Ret. Soln in a steamer for 40 minutes
Incubate 15 min with it at the dilution of 1:25.
Then I use Dako LSAB2 kit.
It works fine.
Good
Has anyone experience edge affect on TMA when doing ISH?
Dana Settembre
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Kathleen Dittrich, formerly from Dako.
Where are you?
Dana Settembre
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Does anyone experience edge affect on TMA?
Dana Settembre
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Are you staining by hand or are you automated?
Dana Settembre
University Hospital
Newark, NJ
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sris...@mail.holyname.org
Sent: Thursday, October 07, 2010
Hi Michele,
Yes, it's true, only MTM labs sells it and they
Sell it as a kit and they also
sell it along with a negative control reagent.
That's how I buy it.
They have a license or something and no else can sell it now.
Their cat# is 9518 and I think it's called the
CINteck Histology V-kit,
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