Hi everyone,
We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin
following Oil Red O staining. We cannot get hematoxylin staining to work. We
are mainly seeing blue background but not labeling of nuclei.
Tissue is fixed with 4% PFA prior to sectioning. After tissue is
Hello,
Has anyone ever used SDS along with trition in ICC protocols?
We see very different results when we use Triton alone without SDS.
Thanks!!
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You might have some luck trying this -
Wipe out the cryostat with a dryer sheet to remove the static. This trick works
for me alot. Sometimes you need to repeat it a few times during cutting. Wiping
the cold plate withthe dryer sheet works wonders for me!
Good Luck!!
Stacey
--- On Fri,
Hi everyone,
What brand low profile blades does everyone prefer and where do you order them?
Also, what blade angle does everyone prefer for cryosectioning?
Thanks
Stacey
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Hi Maria
I will send the manual to you in a few mins as a pdf.
Stacey
--- On Mon, 2/16/09, maria.benll...@uv.es maria.benll...@uv.es wrote:
From: maria.benll...@uv.es maria.benll...@uv.es
Subject: [Histonet] manual of a Nikon Diaphot 300
To: histonet@lists.utsouthwestern.edu
Date: Monday,
Necat,
I have just sent you the pdf from my other email address. Let me know if it
doesnt go through.
Have a great weekend!
--- On Fri, 11/14/08, Nejat Yilmaz [EMAIL PROTECTED] wrote:
From: Nejat Yilmaz [EMAIL PROTECTED]
Subject: [Histonet] Nikon Diaphot 300
To:
