Does anyone have any experience with processing tissues with silicone mesh
in them so that the silicone is not dissolved by xylene and we also assume
that it might be dissolved by MMA or GMA polymers since they are strong
solvents in their own right?
Thank you,
Patsy
Patsy Ruegg,
Lester,
Probably not in most cases but it depends upon what the antibody is, whether
as a an antihuman ab it also cross reacts to the species you are interested
in and what detection reagents you are using.
For instance if you have determined a rabbit anti human antibody also cross
reacts in
It can be confusing because people use different words to describe, we call
picking up every section serial sectioning and the distance between
sections for levels is determined by the requestor in our case, so they
might ask for six more levels to be cut with 50 microns thrown out between
each
Dear Colleagues,
If you are attending USCAP and get a chance take a look at this poster.
A couple of years ago I was involved in an IHC HIER study to compare work
done near sea level to work done at an altitude above 5K feet, it is the
first work of this kind since I wrote an article about
Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti
human cd68.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
-Original Message-
From:
Amos u might have to get an anti rabbit link that has been mouse absorbed.
Dako's anti rab envision is made in a goat but it is not mouse absorbed.
Southern Biotec or Jackson Labs are really good places to get absorbed
links. Still no reliable cd4 and cd8 for ffpe mouse tissue as far as I
know, I
I agree with what Liz has said. Usually when I encounter undesirable bg
staining especially if it is nuclear it most often points to over retrieval
of tissues not adequately fixed. Besides using less harsh retrieval such as
low ph HIER, EIER, or no IER Loui Harkin is a big believer of prefixing
We use the Leica platforms and just got a Leica RX which is for animal research
and ISH, we love the openness of it.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
rueggihcconsultin...@outlook.com
Very interesting post and life Ray, you should be proud of your accomplishments
and contributions. I agree and and share your concerns about our inadequate
STEM training for our up coming generations. Carl Sagan once said our
politicians and many inadequately trained teachers have a vested
i would think u are correct in advising formic acid decal and then processing
into paraffin for the best protection of the trap enzyme, immunoreactivity,
etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be
the same as formalin. I do know a way to restore the enzyme
I would think so,
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
rueggihcconsultin...@outlook.com
This email is confidential and intended solely for the use of the Person(s)
('the intended
This is a tuff one CD34 will stain all precursor cells not just endothelial
cells for vessels, there is an excellent CD31 for ms tissue from Dionova it is
rat anti ms but not sure of what you could use for cd31 for rat and rabbits,
but I know cd34 is not the way to go.
Patsy Ruegg,
Years ago/maybe more than 25 we used a faxitron xray machine to measure bone
calcification on research samples and samples being decaled, it was the best
thing since sliced bread, had to go to the radiology dept to use it and was
told they were dying off the market, loved it, so easy to use and so
I agree with Barbara try fixing in cold acetone/ethanol mix even = if
just for a couple of minutes then go directly to buffer do not dry
after= fix, I would dilute the protease 1:4 with buffer (PBS or TBS
what ever u u= se) and do it for a short time. Enzyme digestion on
do u see this question posted?
Original Message = br
Subject: [Histonet] RE:
Submitting Questions
From: Martha Ward-= Pathology [1]mw...@wakehealth.edu= /a
Date: Tue, September 24, 2013 12:54 pm
To: Nails, Felt= on
Can someone help Dr Cartun make sure he is registered with Histonet?
He sent me this message:
Our e-mail addresses recently changed here at Hartford Hospital. I sent an
e-mail to Histonet with Subscribe in the Subject line, but I don't know
if it was accepted. His new email address is
Does anyone know where I can get FFPE spleen tissue from rabbit, hamster,
and guinea pig?
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
mailto:prueg...@hotmail.com prueg...@hotmail.com
Colleen,
The antigen expression you are getting in the human tonsil may not be
expressed in the mouse femur, plus u have to make sure the ab u are using
cross reacts with mouse tissue, if it is anti human it may or may not react
in mouse, the ab data sheet should indicate species reactivity. The
Good idea. I have to soak the edta decaled tissues in an alkaline solution
inorder to restore enzyme histochemical staining for TRAP, might be the same
issue for some IHC.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
Can someone direct me to a protocol for processing eyes which have been
injected with Davidson's fixative and are now in 10% NBF. We had an eye
expert at the U for years I was going to send my techs to but apparently she
has finally retired because we cannot get a hold of her. Where are you
Can anyone recommend a good antibody made in rat that works in mouse tissue
that is a nuclear marker and another one that is a membrane marker?
Can anyone recommend a good antibody made in goat that works in mouse and
rat tissue that is a nuclear marker and another one that is a membrane
Beware of invitations to speak at an OMICS conference, check out this link
for details
http://scholarlyoa.com/2013/01/25/omics-predatory-meetings/
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
Does anyone know what this is about? I think these people just want a
trichrome stain and we are already doing a Masson's TC for them using
aniline blue. I think they are just asking for a modified version of TC and
want to make sure we use aniline blue for the collagen stain??? Is Mallory
Has anyone tried this: zinc salt fixed mouse tissue PE stained for CD4/cd8
I found some papers that look impressive.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
mailto:rueggihcconsultin...@outlook.com
Is this still being done on frozen sections not aldehyde fixed or has
someone figured out an antibody or method of doing it on FFPE mouse tissue
yet???
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
The most important part= about working up a new antibody is to have
known positive tissue to use as= a control, if you do not have that
everything you do is a shot in the dark= , you will not know if your
protocol is not working or the control you are = using is not
There is nothing volunteer about being ASCP certified as an HT or= HTL
where I have worked for the last 35 years, all those employed as HT's
= at the U of Colorado must be ASCP certified and I believe this is
the case = for most other places doing hospital based Histology, work,
Well said Will.
Original Message
Subject: Re: [Histonet] Unregistered techs
From: William Chappell = a
href=mailto:cha...@yahoo.com;cha...@yahoo.com
Date: Thu, M= ay 24, 2012 4:02 pm
To: Davide Costanzo [1]pathloc...@gmail.com
Cc:
i use TGFb from Peprotech
Original Message -= ---
Subject: [Histonet] TGF beta
From: Houston, Ronald Ronald.Houston@nation= widechildrens.org
Date: Sat, October 22, 2011 8:19 am
To: [1]histonet@lists.utsouthwe= stern.edu
[2]=
I have seen this done many ways, some people have h202 at th= e end of
their depara setup if doing that off line. For the Leica Bon= d
instrument we were advised to do h202 block just before the DAB and i
hav= e noticed that it works best that way for their system. I for
that is a very interesting thread. I am a senior histotech = but i
own my own Histopathology Services Business so i get to decide w= hat
everyone does including me. I tend to do most of the QC/QA work a= nd
management these days but i do not hesitate to work at the bench when
I do not melt dry my cut control slides until i need to use them,= and
i do store them in boxes at 4c, i have recently run into this problem
= with blocks, so now i seal all my blocks with paraffin and store
them in th= e fridge as well.
Patsy
Original
Anjan,
I am more of a fan of steamer or waterbath than pressure cooker, in
my= hands at altitude the pressure cooker can be very harsh and make
my tissue= s fall off the slide or get chewed up badly. I know the
pressure is s= upposed to be controled by the cooker but in
Subject:
respond directly to Tim on this atnbs= p;[1]tmal...@cox.net
HELP ! HISTONET ADDRESS
From:
Timothy Malloy [2]tmal...@cox.net ([3]Add as Preferred Sender)
[4] 3D?
= /TD
Date: Thu, Dec 11, 2008 2:57 am
To: [5]pru...@ihctech.net, [6]tmallo...@hotm=
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