Re: [Histonet] Alcian blue van gieson double stain

2023-03-09 Thread Tony Reilly via Histonet
Hi Charles I have done this stain many times as an abbreviated version of Movats Pentachrome. You are correct in thinking the van gieson will affect the AB stain due to the acid. As in the Movat stain do AB first then place slides in alcoholic ammonia for 1 hour to convert AB to insoluble Mona

Re: [Histonet] Alcian blue van gieson double stain

2023-03-09 Thread Akemi Allison via Histonet
Just curious, why not do a Movat’s Pentachome? It gives you everything you are looking for and more. Best regards, Akemi Allison-Tacha, BS, Ht, HTL akemiat3...@gmail.com Sent from my iPhone > On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet > wrote: > > Hi, > if they are wanti

Re: [Histonet] Alcian blue van gieson double stain

2023-03-09 Thread Paula Keene Pierce via Histonet
Hi, if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http:

[Histonet] Alcian blue van gieson double stain

2023-03-09 Thread Charles Riley via Histonet
I am working with a researcher and they saw an article where someone performed an Alcian Blue and elastic van Gieson double stain. If anyone has done this before can you provide your protocol. I am trying to figure out the best way to perform this process to get the best staining results. Here a