Hi Charles
I have done this stain many times as an abbreviated version of Movats
Pentachrome.
You are correct in thinking the van gieson will affect the AB stain due to the
acid.
As in the Movat stain do AB first then place slides in alcoholic ammonia for 1
hour to convert AB to insoluble Mona
Just curious, why not do a Movat’s Pentachome? It gives you everything you are
looking for and more.
Best regards,
Akemi Allison-Tacha, BS, Ht, HTL
akemiat3...@gmail.com
Sent from my iPhone
> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet
> wrote:
>
> Hi,
> if they are wanti
Hi,
if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend
doing the Verhoff elastic stain first, then the Alcian blue, and then the Van
Gieson.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH 405-759-3953http:
I am working with a researcher and they saw an article where someone
performed an Alcian Blue and elastic van Gieson double stain. If anyone
has done this before can you provide your protocol. I am trying to figure
out the best way to perform this process to get the best staining results.
Here a