If you use clone 6E10 it will demonstrate beta Amyloid only, after Formic acid
AR
After Citric acid HIER, APP will also be demonstrated
Imho
Check out Biosource ( Invitrogen) 44-344 and 44-348?
Something about x-reactivity between Abeta 1-42 and Abeta 1-43?
Or not.
I'd be interested to
ichard*
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>
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> *From:* Colleen Forster [mailto:cfors...@umn.edu]
> *Sent:* Wednesday, December 27, 2017 4:23 PM
> *To:* Cartun, Richard
> *Cc:* histonet@lists.utsouthwestern.edu
> *Subject:* Re: [Histonet] Beta-Amyloid antibody for IHC
>
>
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> CAUTION: This ema
No, I would like to use “6E10” if it’s still available. Thank you!
Richard
From: Colleen Forster [mailto:cfors...@umn.edu]
Sent: Wednesday, December 27, 2017 4:23 PM
To: Cartun, Richard
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Beta-Amyloid antibody for IHC
CAUTION
We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed,
paraffin-embedded human brain tissue. Any recommendations? We have been using
the clone "6E10" which we purchased from Signet Laboratories back in 2001.
Thank you!
Richard
Richard W. Cartun, MS, PhD
Director,
Do you knot want to use 6E10 anymore? I believe you can still get it from
Biolegends?
https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228
Respectfully,
Colleen Forster HT(ASCP)QIHC
*University of Minnesota*
On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard