Re: [Histonet] Beta-Amyloid antibody for IHC

2017-12-28 Thread Hobbs, Carl via Histonet
If you use clone 6E10 it will demonstrate beta Amyloid only, after Formic acid AR After Citric acid HIER, APP will also be demonstrated Imho Check out Biosource ( Invitrogen) 44-344 and 44-348? Something about x-reactivity between Abeta 1-42 and Abeta 1-43? Or not. I'd be interested to

Re: [Histonet] Beta-Amyloid antibody for IHC

2017-12-27 Thread Colleen Forster via Histonet
ichard* > > > > *From:* Colleen Forster [mailto:cfors...@umn.edu] > *Sent:* Wednesday, December 27, 2017 4:23 PM > *To:* Cartun, Richard > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Beta-Amyloid antibody for IHC > > > > CAUTION: This ema

Re: [Histonet] Beta-Amyloid antibody for IHC

2017-12-27 Thread Cartun, Richard via Histonet
No, I would like to use “6E10” if it’s still available. Thank you! Richard From: Colleen Forster [mailto:cfors...@umn.edu] Sent: Wednesday, December 27, 2017 4:23 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Beta-Amyloid antibody for IHC CAUTION

[Histonet] Beta-Amyloid antibody for IHC

2017-12-27 Thread Cartun, Richard via Histonet
We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, PhD Director,

Re: [Histonet] Beta-Amyloid antibody for IHC

2017-12-27 Thread Colleen Forster via Histonet
Do you knot want to use 6E10 anymore? I believe you can still get it from Biolegends? https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228 Respectfully, Colleen Forster HT(ASCP)QIHC *University of Minnesota* On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard