I just made the aluminum temple from a 1/2 inch 6x6 piece on aluminum from Home
Depot. With the sheet you will este,Italy make it look like a clock face using
a sharpe. Why you want to do this is i found the aluminum plates have enough
variance that if I did not always use the same side it
Hi Loralei, I will see If I can find someone with a sliding microtome. Are
you using OCT to block the 50 um section? How do you ensure the section stays
flat while blocking/freezing?
Thank you for your reply !
E-van
From: Loralei Dewe [mailto:lld...@gmail.com]
Sent: Friday, October 06, 2017
Hi Robert, thank you for your reply. So you froze the section on the aluminum
with the "clock face" and then put this inverted, in the Microm 560 mold? ours
is an older unit that has been around and not all the parts came with it so I
don't have the 90 degree block chuck. Is this the fixed
HI Ms. Powell!! , Thanks for the reply. When we last all saw each other at the
Georgia Histopalooza I expressed an interest in training on the cryostat with
Cindy Baranowski. She was so nice and had me come over to the hospital back in
June. That was really really nice of her! But now I can't
Haley,
Maybe ask Stephen directly. He is on histonet, and has a website:
https://www.pathologyinnovations.com/
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original
I would also be interested in knowing this tip if anyone knows it. I will
also have to check out that book. We only do cryostat sections at our lab.
*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*
On Fri, Oct 6,
Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from
50um sections of brain. Every time I think I have the tissue flat the sections
are never in the same plane. I found a wonderful book in pdf format(A
Practical Guide to Frozen Section Techniques, Stephen R. Peters)
