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From: Rhonda McCormick via Histonet
Sent: August 30, 2023 2:42 PM
To: Histonet
Subject: [Histonet] Gram Stain
Howdy,Does anyone have a recommendation for a Gram stain (or modification of a
gram stain) that stains the background yellow with red gram-negative bacteria
(no blue - gram positive
Howdy,Does anyone have a recommendation for a Gram stain (or modification of a
gram stain) that stains the background yellow with red gram-negative bacteria
(no blue - gram positive staining).Thank you!
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Dear Histoneters:
If Gram stains are not always working consistently: how likely is it that
crystal violet has gone bad. My first suspect is the decoloration step,
then age of the culture, then thickness of the smear. However, I do not
want to disregard any possibility, such as bad reagents.
We only perform a few Gram stains a year, hardly seems worth It to keep a kit
and expire reagents! Does anyone have an idea for other options? Ie, doing the
stains in Micro? Checking with their
BD rep to see if we can do their Gram stain kit on formalin fixed tissue,...
Tanya G. Abbott RT
I do a gram stain but also not very often. I have used a PolyScientific kit and
it has a pretty good shelf life but before that I was just making up the stains
and solutions in small quantities. They aren't hard to make and I inherited a
wealth of stains and chemicals here that it seems a shame
Hi All,
Does anybody have a protocol for Gram stain on FFPE mouse intestine
sections. We are looking to distinguish between Klebsiella and Vancomycin
Resistant Enterococcus (VRE). I would like to avoid using Picric acid as
some protocols suggest.
Thanks in advance.
We have had great success with the Twort gram stain.
From: Mesru T turke...@gmail.com
To: histonet@lists.utsouthwestern.edu
Date: 10/02/2013 10:13 AM
Subject:[Histonet] Gram stain
Sent by:histonet-boun...@lists.utsouthwestern.edu
Hi All,
Does anybody have
I'm having some QC issues lately with my gram stain. The gram positive
bacteria on the control slide are only mostly gram positive. It seems
positive in some areas and negative in others. It's not like only the
higher portions are positive either, it's patchy. When I differentiate
dipping the
Hi histonetters
does any one have a good working method for GRAM stain ???
I used mod brown's variation and my Neg bact still does not stain red.
Fawaz Zouabi
Histo-Technologist
Department of Forensic Medicine Glebe NSW Forensic Analytical Science
Service - FASS
P O Box 90 Glebe NSW 2037 |
Subject:[Histonet] Gram stain
Sent by:histonet-boun...@lists.utsouthwestern.edu
Hi histonetters
does any one have a good working method for GRAM stain ???
I used mod brown's variation and my Neg bact still does not stain red.
Fawaz Zouabi
Histo-Technologist
Department of Forensic
I have a pathologist that wants a gram stain on decalcified bone. We are
currently using the Twort's gram stain and the stain did not stain the bacteria
in the bone sample, but the control tissue did stain positive. I was wondering
if the acid in the decal solution could have affected the
Yes, they have been affected.
René J.
From: Shelly Christenson chris...@vet.k-state.edu
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, February 26, 2013 4:11 PM
Subject: [Histonet] Gram stain on decalcified bone
I have a pathologist that wants a gram
Hi,
Our lab is currently transitioning from the Brown Brenn gram stain due to no
longer wanting to store picric acid due to its potential hazards. Our
pathologists have requested a gram stain for paraffin embedded tissue that
looks similar but does not use picric acid or ether. Does anyone
@lists.utsouthwestern.edu
Subject: [Histonet] Gram Stain
Hi,
Our lab is currently transitioning from the Brown Brenn gram stain due to
no longer wanting to store picric acid due to its potential hazards. Our
pathologists have requested a gram stain for paraffin embedded tissue that
looks similar but does not use picric
Does anyone have a method for the Twort stain or a gram stain that does
not use picric acid or ether? We were using a Twort stain and I cannot
find the original reference for it and we suspect we have one of the
concentrations wrong.
Thank you,
Jennifer MacDonald
Thank you!!
Tony Henwood antho...@chw.edu.au
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/24/2011 04:54 PM
To
'Jennifer MacDonald' jmacdon...@mtsac.edu,
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc
Subject
RE: [Histonet] gram stain
Jennifer,
Here
HI all,
We are doing gram stains manually, and the acetone is removing some of the
'ink' from the printed labels making them difficult to read--let alone scan the
barcode. Does anyone have any suggestions/hints on what they are using for
their gram stain that may lessen the acetone removal of
Original Message
Subject: [Histonet] gram stain/label issues
From: Jacqueline Farnsworth [1]jacqueline.farnswo...@cls.ab.ca
Date: Tue, October 26, 2010 1:56 pm
To: [2]histo...@lists.ut= southwestern.edu
[3]histo...@lists.uts= outhwestern.edu
HI all,
We
.
---Original message---
Date: Fri, 23 Apr 2010 16:25:30 -0500
From: Perry, Margaret margaret.pe...@sdstate.edu
Subject: [Histonet] Gram stain
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
fca5ef47f9bc694cbb4c58fea04219634ccfb69...@sdsu
Now that I'm done with my rant I have a real question. We are trying to do a
gram stain on fish and the safranine O is staining everything red. What other
stain would you use? I usually have time to look at the books but
unfortunately it's almost quiting time and the slides need to be done
Can anyone recommend a good gram stain kit? We currently do a modified
Brown-Hopps stain and make up all of the reagents, but I am looking for
a kit.
Laurie Colbert
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: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa
Sent: Tuesday, November 25, 2008 3:40 PM
To: histonet@lists.utsouthwestern.edu; Fischer, R. B
Cc: Delcambre, Linda V
Subject: Re: [Histonet] gram stain controls
I always used actual tissues with gram +/- bacteria because
My pathologist has made a gram neg/pos control block for us to use.
He made this by taking separate cultures of each and suspended them in
histo gel. The control stains well. My question is: Since we are
performing gram stain on tissues exclusively, will this cultured control
be an accepted
I always used actual tissues with gram +/- bacteria because it is not the same
(time wise) to stain a histogel than a piece of tissue. Appendix is a good
control.
René J.
--- On Tue, 11/25/08, Fischer, R. B [EMAIL PROTECTED] wrote:
From: Fischer, R. B [EMAIL PROTECTED]
Subject: [Histonet] gram
Hello!
To introduce myself: I've been reading this list for the last ~350 mails. I am
a technical assistent, working in vet histo.
I am quite inexperienced with staining, and one task is to get Gram staining to
work. Right now, I've been trying to get results for 2! days, I tried about 10
Valentin:
Later in the day I will send you my procedure. It always worked very well for
me.
René J.
--- On Fri, 11/7/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote:
From: [EMAIL PROTECTED] [EMAIL PROTECTED]
Subject: [Histonet] Gram stain on tissue
To: histonet@lists.utsouthwestern.edu
Date
Thank you for all replies.
Just a quick update: I have carbol-gentiana-violet, of course, I just forgot to
mention it.
Please tell me how to decolorize the fuchsin without affecting the violet, that
would help me very much in this moment. Thank you!
I'll post my results and my method of
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