>
> In answer to a query about tissue Gram staining, Tony Henwood adds
> instability of the iodine solution to the long list of reasons why tissue
> Gram staining doesn't work very well.
>
The Gram stain done on smears is of course one of the most useful
microtechniques in microbiology, but tissu
en's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: Ken M via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, 1 July 2020 7:21 AM
To: histonet@lists.utsouthwestern.edu
Sub
Hi.
We are trying to stain a supposed Gram Positive paraffin Tonsil tissue block
using Gentian Violet, Iodine (Lugol), Gram's decolorizer, Carbol Fuchsin
Counter and Tartrazine and are having trouble distinguishing the positive from
the negative. Anyone have any suggestions?
_
Our new Pathologist would like to see more yellow in the background of our Gram
stain. We use picric acid-acetone currently, which looks more pink than yellow
most times. So I'm wondering if a method using a Tartrazine counterstain would
be better.
Any thoughts?
IMPORTANT WARNING: The infor
