4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Andrea
Hooper
Sent: Friday, 5 December 2008 3:12 AM
To: FU,DONGTAO; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC on fresh frozen
Try fixing for 5-10 min
: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]
Sent: Friday, 5 December 2008 1:31 AM
To: Tony Henwood; Jan Shivers; histonet
Subject: Re: [Histonet] IHC on paraformaldehyde-fixed
So true. However, be aware that 10% neutral buffered formalin we use has
methanol in it which may affect certain antigens so
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]
Sent: Friday, 5 December 2008 1:31 AM
To: Tony Henwood; Jan Shivers; histonet
Subject: Re: [Histonet] IHC on paraformaldehyde-fixed
So true. However, be aware that 10% neutral buffered formalin we use has
methanol in it which may
stable, both for storage and transportation~~~.
2008-12-05
tf
发件人： Tony Henwood
发送时间： 2008-12-05 06:00:03
收件人： [EMAIL PROTECTED]; Jan Shivers; histonet
抄送：
主题： RE: [Histonet] IHC on paraformaldehyde-fixed
Interesting point.
Since 10% buffered formalin (made from the concentrated 38
]
Sent: Friday, 5 December 2008 2:11 PM
To: Tony Henwood; [EMAIL PROTECTED]; Jan Shivers; histonet
Subject: Re: RE: [Histonet] IHC on paraformaldehyde-fixed
the basic principles are the same for most cross-linking fixatives and
induce similar bonds
Hi, all
Recently I did some IHC(chromagen methods) on mouse fresh frozen
tissues, mainly using insulin antibody on pancreas. The image is
much fuzzier compare to paraffin embedding tissue. And the
staining also smeared to acinar cells which surround the islet.
I airdried slide(30min) and
:[EMAIL PROTECTED] On Behalf Of
FU,DONGTAO
Sent: Wednesday, December 03, 2008 9:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC on fresh frozen
Hi, all
Recently I did some IHC(chromagen methods) on mouse fresh frozen
tissues, mainly using insulin antibody on pancreas
After hearing a presentation by Sharon Lear describing some low temp antigen
retrieval that she did, we changed our method for frozen sections. We fix
the frozen sections in 10% nuetral buffered formalin for 30'-60', rinse,
then do a gentle pretreatment. The gentle pretreatment is usually 10mM
I do it all the time. Yes your antigen-retrieval method will work.
Ruth
N.I.H.
-Original Message-
From: Jan Shivers [mailto:[EMAIL PROTECTED]
Sent: Wednesday, December 03, 2008 4:34 PM
To: histonet
Subject: [Histonet] IHC on paraformaldehyde-fixed
Has anyone ever done IHC
] On Behalf Of Jan
Shivers
Sent: Thursday, 4 December 2008 8:34 AM
To: histonet
Subject: [Histonet] IHC on paraformaldehyde-fixed
Has anyone ever done IHC on parafomaldehyde-fixed tissues, and if so,
how well did it work? Will the same antigen-retrieval methods used with
formalin-fixed tissue
Any info would be appreciated. Thanks in advance.
Larry A. Woody
Seattle, Wa.
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-6596
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Larry Woody
Sent: Thursday, November 06, 2008 11:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC for helicobacter bili in mouse gut?
Any info would be appreciated. Thanks in advance
I am helping to set up an in office lab for a group of urologists. What is the
best instrumentation for prostate tripple stain? What are the best reagents?
Test volume will be relatively low. Perhaps as many as 5 additional
immunostains will be added in the future. Testing will be done by
anyone out there that has a ventana benchmark XT, how do you handle the
reports for the pathologist to sign off on when you have more than one path?
do you just print one for each dr. or do then pass it around and sigh off on
their case?
we had a joint com. person tell us that we could not
Dear all,
I've done the IHC staining step unfortunately encounter problem for a good
scoring system (i.e scoring that balance between cell distribution and staining
intensity).
Hope anyone who have experience in this matter can share they're opinion.
Thank you in advance.
Cheers,
Shazana
Birmingham, AL 35213-1984
[EMAIL PROTECTED]
205-592-5387 (office)
205-592-5388 (lab)
205-592-5646 (fax)
Message: 3
Date: Wed, 15 Oct 2008 11:56:21 -0700
From: Laurie Colbert [EMAIL PROTECTED]
Subject: [Histonet] IHC for Amyloid L
To: histonet@lists.utsouthwestern.edu
Message-ID
Does anyone know of a reference lab that does an IHC stain for Amyloid L
(amyloid light chain)? I can find labs that do Amyloid A and Amyloid P,
but not Amyloid L.
Laurie Colbert
Huntington Hospital
Pasadena, CA
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Folks,
I have been asked to post this question about continuous feed batching for
IHC stainers:
continuous feed stainers - Bond is really the only one at 10 slides per
batch, though the old Benchmark was similar at 20 per batch. The new
Biocare is 12 per batch. We are wondering if people
--- On Fri, 10/10/08, Poteete, Jacquie A. [EMAIL PROTECTED] wrote:
From: Poteete, Jacquie A. [EMAIL PROTECTED]
Subject: RE: [Histonet] IHC antibody Expiration question for CAP
To: [EMAIL PROTECTED], histonet@lists.utsouthwestern.edu
Date: Friday, October 10, 2008, 7:32 AM
We are not permitted to use
:32
To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC antibody Expiration question for CAP
We are not permitted to use anything that is expired, no matter what it
is. Yes, the lab takes a major hit because of this regulation. I send
my expired antibodies to Patsy
Francis Hospital
Tulsa, OK
-Original Message-
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:[EMAIL PROTECTED]
Sent: Friday, October 10, 2008 10:07 AM
To: Poteete, Jacquie A.; [EMAIL PROTECTED];
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC antibody
PROTECTED] On Behalf Of Poteete,
Jacquie A.
Sent: Friday, October 10, 2008 10:18 AM
To: Marshall Terry Dr,Consultant Histopathologist;
[EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC antibody Expiration question for CAP
I agree, it is a very ridiculous rule, and I
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