Re: [Histonet] IHC Stainer

2018-08-30 Thread Colleen Forster via Histonet
ge- > From: Sandra Cheasty [mailto:sandra.chea...@wisc.edu] > Sent: Thursday, August 30, 2018 9:40 AM > To: Histonet (histonet@lists.utsouthwestern.edu) utsouthwestern.edu> > Subject: [Histonet] IHC Stainer > > Hello all, > We are in the market for a new IHC stainer. (O

Re: [Histonet] IHC Stainer

2018-08-30 Thread Jennifer Kempf via Histonet
as well, instead of in the pressure cooker, but I haven't seen it in action yet. -Original Message- From: Sandra Cheasty [mailto:sandra.chea...@wisc.edu] Sent: Thursday, August 30, 2018 9:40 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Stainer Hello all

Re: [Histonet] IHC Stainer

2018-08-30 Thread Rene J Buesa via Histonet
Get a DAKO IHC stainer (even if it is "refurbished")René On Thursday, August 30, 2018 9:56 AM, Sandra Cheasty via Histonet wrote: Hello all,                 We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but

[Histonet] IHC Stainer

2018-08-30 Thread Sandra Cheasty via Histonet
Hello all, We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides. Not interested in a closed system; it is for a veterinary histology lab, and we need the

[Histonet] IHC Stainer Dispense volume

2013-03-21 Thread Tiana Baskin
I currently use a Dako Autostainer and we are dispensing 300uL of all reagents to the slide(100uL per drop zone). I am just curious what others are dispensing and if you've noticed any difference by changing the dispense volume in regards to stain quality. Does anyone adjust for very large

RE: [Histonet] IHC Stainer Dispense volume

2013-03-21 Thread Milne, Katy
Hi Tiana, We just got a Biocare Intellipath FLX and it came set at 300ul too. I've switched it to 150ul, dropping in the middle zone only and had no problems, may see if I can go 100ul for smaller tissues, will up it for larger tissues. I pap pen around the samples though to make sure that

RE: [Histonet] ihc stainer

2010-03-05 Thread Rathborne, Toni
To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ihc stainer We are a small lab with a low volume and we are thinking about beginning to do our own ihc's. Can anyone recommend an ihc stainer that would be suitable for a small lab? ___ Histonet

[Histonet] IHC stainer

2009-07-03 Thread Gomez, Milton
Dear Histonetters: What is the best, most reliable workhorse immunostainer out there nowadays? and why. Thanks in advance, MG - -- The information transmitted by this e-mail and any included attachments are from ARUP

[Histonet] IHC stainer- Nemisis

2009-06-26 Thread sheila adey
Hello Netters, Our lab is thinking about purchasing a Nemisis IHC stainer, any opinions about this stainer??? Thanks in advance. Sheila Adey HT MLT _ Attention all humans. We are your photos. Free us.

[Histonet] IHC Stainer: Microm HMS 710i

2009-03-24 Thread Katelin Lester
I am looking for help/advice for using our automated immunostainer the Microm HMS 710i .(Our user manual says Richard Allan Sci and the labels on the side say LabVision, we have calls into Thermo's LabVision department but they are unsure if they can assist us) Specifically, one of our problems

RE: [Histonet] IHC stainer

2009-03-16 Thread Bower, Jennifer
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, March 15, 2009 10:05 AM To: 'Pamela Marcum'; 'Greg Dobbin' Cc: histonet@lists.utsouthwestern.edu; swea...@tvmdl.tamu.edu; 'Ronald Houston' Subject: RE: [Histonet] IHC stainer

RE: [Histonet] IHC stainer

2009-03-15 Thread Patsy Ruegg
; swea...@tvmdl.tamu.edu; Ronald Houston Subject: Re: [Histonet] IHC stainer Just be sure it is an open system that will allow you to use any primary antibody you need and secondary antibody not just a secondary kit the company provides. You will need to confirm that if you are using a chicken

[Histonet] IHC stainer

2009-03-13 Thread Stephanie Weaver
I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience

RE: [Histonet] IHC stainer

2009-03-13 Thread Houston, Ronald
: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Friday, March 13, 2009 3:17 PM To: histonet post Subject: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC

Re: [Histonet] IHC stainer

2009-03-13 Thread Greg Dobbin
I echo everything Julie said! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have.

RE: [Histonet] IHC stainer

2009-03-13 Thread Greg Dobbin
: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none

Re: [Histonet] IHC stainer

2009-03-13 Thread Pamela Marcum
:57:48 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] IHC stainer I guess I would have to echo that as well! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902