Hi Tiana,
We just got a Biocare Intellipath FLX and it came set at 300ul too. I've
switched it to 150ul, dropping in the middle zone only and had no problems, may
see if I can go 100ul for smaller tissues, will up it for larger tissues.
I pap pen around the samples though to make sure that rea
I currently use a Dako Autostainer and we are dispensing 300uL of all reagents
to the slide(100uL per drop zone). I am just curious what others are dispensing
and if you've noticed any difference by changing the dispense volume in regards
to stain quality. Does anyone adjust for very large tissu