Hi Charles- IHC staining - We also use the Leica Bond staining system and had tissue fall off in the beginning. We blot the slides to remove any additional water and then place in drying oven for 30 minutes at 60 before placing on the stainer.
Water baths - at the end of each day the water is dumped, the bath is rinsed with bleach solution, rinsed with water and dried. Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager -----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, February 26, 2020 9:44 AM To: Histo List Subject: [Histonet] IHC troubleshooting EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs Message: 5 Date: Wed, 26 Feb 2020 17:09:14 -0500 From: Charles Riley <cri...@dpspa.com> To: Histo List <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Poll question Message-ID: <CAAQhB11Kt6gG7XLVcdbSpUwpZOL1fo=q5q6aqt1jkhmk3x8...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" How often does everyone clean their water-bath? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
