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-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Andrea
Hooper
Sent: Friday, 5 December 2008 3:12 AM
To: FU,DONGTAO; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC on fresh frozen
Try fixing for
Try fixing for 5-10 min in PFA.
At 10:16 AM -0500 12/3/08, FU,DONGTAO wrote:
Hi, all
Recently I did some IHC(chromagen methods) on mouse fresh frozen
tissues, mainly using insulin antibody on pancreas. The image is
much fuzzier compare to paraffin embedding tissue. And the staining
also sm
e, MA
From: Patti Loykasek <[EMAIL PROTECTED]>
To: "FU,DONGTAO" <[EMAIL PROTECTED]>; histonet@lists.utsouthwestern.edu
Sent: Wednesday, December 3, 2008 11:38:07 AM
Subject: Re: [Histonet] IHC on fresh frozen
After hearing a presentation by Sharon Lear descr
After hearing a presentation by Sharon Lear describing some low temp antigen
retrieval that she did, we changed our method for frozen sections. We fix
the frozen sections in 10% nuetral buffered formalin for 30'-60', rinse,
then do a gentle pretreatment. The gentle pretreatment is usually 10mM
citr
ilto:[EMAIL PROTECTED] On Behalf Of
FU,DONGTAO
Sent: Wednesday, December 03, 2008 9:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC on fresh frozen
Hi, all
Recently I did some IHC(chromagen methods) on mouse fresh frozen
tissues, mainly using insulin antibody on pancreas.
Hi, all
Recently I did some IHC(chromagen methods) on mouse fresh frozen
tissues, mainly using insulin antibody on pancreas. The image is
much fuzzier compare to paraffin embedding tissue. And the
staining also smeared to acinar cells which surround the islet.
I airdried slide(>30min) and
