[Histonet] RE: Question

2015-04-30 Thread Morken, Timothy
Bernice, You have to buy the 10N solution. You can only dilute a given normal solution, you cannot concentrate them. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

RE: [Histonet] RE: Question on IHC billing

2015-04-24 Thread Joelle Weaver
: Subject: [Histonet] RE: Question on IHC billing Correct Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments

[Histonet] RE: Question about Formic acid decal and TRAP stain

2015-04-02 Thread Elizabeth Chlipala
Debbie In our hands it has not worked on formic acid decaled samples, EDTA decal only. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March

[Histonet] RE: Question about POC testing

2015-02-20 Thread Wanda.Smith
Cytology here. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC  29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only

[Histonet] RE: Question about POC testing

2015-02-20 Thread Hannen, Valerie
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Friday, February 20, 2015 9:23 AM To: caroline.pr...@uphs.upenn.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Question about POC testing Cytology here. WANDA G

[Histonet] RE: Question about Lyme Disease

2013-10-25 Thread Cartun, Richard
In my experience, it's not worth looking at tissue for the spirochete using histochemical stains or IHC. There are labs that do PCR off FFPE-tissue. Has serology been done? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

[Histonet] RE: Question about fixation terminology

2013-10-03 Thread Sarah Dysart
http://www.ihcworld.com/_protocols/general_ICC/fixation.htm This article specifically addresses the differences. You are right about the cross linking, but alcohol and acetone are still considered fixatives. Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics

[Histonet] Re: Question about sectioning brain tissue at 10-15 microns

2013-01-31 Thread Teri Johnson
Hi Christina, First I would have you use a room temperature block. If the tissue needs to be rehydrated briefly, use room temp water instead of ice. I can get 12 micron ribbons of bone using Paraplast extra, I haven't tried thicker. If that doesn't work, what blade are you using? In my

[Histonet] Re: Question

2013-01-25 Thread Tim Higgins
All I have to go by is CAP's response to the question, Is IHC a High Complexity Test. Their verbal answer is no, but there is nothing formally documented. The only documented statement is from 2010 Webinar during the Question and Answer portion. It is as follows, Laboratory Accreditation

RE: [Histonet] Re: Question

2013-01-24 Thread Sullivan, Beatrice
Tarango Sent: Wednesday, January 23, 2013 4:20 PM To: Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pie...@quintiles.com Subject: Re: [Histonet] Re: Question If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out

RE: [Histonet] Re: Question

2013-01-24 Thread histotech
] On Behalf Of Sullivan, Beatrice Sent: Thursday, January 24, 2013 7:57 AM To: Mark Tarango; Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pie...@quintiles.com Subject: RE: [Histonet] Re: Question I called CAP a short time ago about who was qualified to run these. I was told

Fw: [Histonet] Re: Question

2013-01-24 Thread Rene J Buesa
AM Subject: RE: [Histonet] Re: Question It also depends on the State you are in.  Florida considers this a high complexity test and requires that a licensed technologist either do the test or oversee a licensed technician who is performing the test. From my view, especially with all the automatic

Re: [Histonet] Re: Question

2013-01-24 Thread Rene J Buesa
AM Subject: RE: [Histonet] Re: Question It also depends on the State you are in.  Florida considers this a high complexity test and requires that a licensed technologist either do the test or oversee a licensed technician who is performing the test. From my view, especially with all the automatic

RE: [Histonet] Re: Question

2013-01-24 Thread Pratt, Caroline
...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2013 12:43 PM To: histot...@imagesbyhopper.com; 'Sullivan, Beatrice'; 'Mark Tarango'; 'Tim Higgins' Cc: histonet@lists.utsouthwestern.edu; courtney.pie...@quintiles.com Subject: Re: [Histonet] Re: Question We have to remember

[Histonet] Re: Question

2013-01-23 Thread Tim Higgins
The professional interpretation is considered a high complexity test but not the actual technical component. Thanks, Timothy N. Higgins, HT (ASCP), QIHC ___ Histonet mailing list

Re: [Histonet] Re: Question

2013-01-23 Thread Mark Tarango
If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there. I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab director is threatening their job saying they aren't qualified to do IHC

[Histonet] Re: Question on frozen section IHC and tissue adhesion

2012-11-20 Thread Teri Johnson
Hi Christina, I agree with Rene Buesa that ideally you should have added a cryoprotection step prior to freezing. If you snap freeze quickly enough though, you might have gotten away with no freezing artifact. An HE will tell you that. If it looks like a sponge, or like frost patterns, you

[Histonet] RE: question on H pylori

2011-06-08 Thread Dawson, Glen
Lisa, We use both IHC and Warthin Starry here, depending on the Pathologist's preference. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] Re: question on H pylori

2011-06-08 Thread Robert Richmond
On the question of staining Helicobacter pylori: There is no standard of care (remember that standard of care is a term of art and should not be used casually) for staining Helicobacter. As far as I know, nobody has ever done a study comparing the efficacy of the various staining methods. Blue

[Histonet] RE: question on H pylori

2011-06-07 Thread Blazek, Linda
IHC here Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: question on H pylori

2011-06-07 Thread Wanda.Smith
IHC on all gastric biopsies here. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read

RE: [Histonet] RE: question on H pylori

2011-06-07 Thread Matt Brooks
...@hcahealthcare.com Sent: Tuesday, June 07, 2011 1:10 PM To: lset...@childrensmemorial.org; histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] RE: question on H pylori IHC on all gastric biopsies here. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor

[Histonet] RE: Question about acid phosphatase staining on FFPE and control tissue

2010-12-06 Thread Thurby, Christina
Hi, We are going to try acid phosphatase staining on FFPE cardiac tissue (I have a couple of papers where this has been performed with success). My question for any of you with expertise in this area is what type of tissue should I use as a Method Development control? Would there be acid

[Histonet] RE: Question about DAB waste

2009-05-13 Thread Smith, Allen
We keep our DAB waste in a plastic container and give it to our waste disposal company every 6 months. No one has objected to this procedure. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From:

RE: [Histonet] RE: Question about DAB waste

2009-05-13 Thread Weems, Joyce
To: Smith, Allen; Jennifer Campbell Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Question about DAB waste On this subject at our recent CAP inspection a question came up about the labeling of the waste containers that held the DAB waste from our Ventana stainer. We had the container

[Histonet] Re: Question about cell buttons

2008-10-24 Thread Robert Richmond
Larry A. Woody in Seattle WA asks: Does anyone out there know what the material is that is used to make cell buttons in cytology and where to order it? I suppose you're referring to the embedding gel for cytology cell blocks (often done with pleural and peritoneal fluids). It goes by the trade