Re: [Histonet] Reconstituting antibodies and dilutions

2022-10-13 Thread Gudrun Lang via Histonet
matter. Gudrun Lang -Ursprüngliche Nachricht- Von: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Donnerstag, 13. Oktober 2022 17:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Reconstituting antibodies and dilutions When calculating

[Histonet] Reconstituting antibodies and dilutions

2022-10-13 Thread Charles Riley via Histonet
When calculating the dilution of an antibody when do start? i.e. If i have 100 ug of lyophilized antibody and its recommended reconstitution is 500 ug/ml How do I mix a 1:1000 dilution? I am thinking it's adding 2mL diH20 to create the 500 ug/mL and then diluting the sample. And I would be