[Histonet] glutaraldehyde perfusion

2009-04-02 Thread Boma Fubara
Can someone explain to me the mechanism by which glutaraldehyde would disrupt the blood brain barrier if the brain had not been flushed prior to fixation? Boma. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] glutaraldehyde perfusion

2009-04-02 Thread Geoff McAuliffe
Hi Boma: Whether or not a saline or buffer flush was used before glut. fixation is of little consequence to the effects of glut as a fixative.The saline/buffer washout is just to remove blood. In my experience most people overdo the washout. I am curious as to what disrupt the blood-brain