Hi,
One mm thick is tiny, 2 mm small 1 cm is rather thick. Since you are
manually processing run one sample to test your protocol or 2 if you want
to see the difference between the shorter method and the longer. *protocol.
.Complete dehydration is dependent on the volume, type of tissue and
I agree with Renee here about testing one before. However those are thick and
I'm supposing your fish have scales? This also might slow the process down. My
best guess is you're going to need a much longer processing time. And since you
don't have vacuum I personally think that thickness is
Thanks
On Thu, Feb 27, 2014 at 5:50 PM, Kim Donadio one_angel_sec...@yahoo.comwrote:
I agree with Renee here about testing one before. However those are thick
and I'm supposing your fish have scales? This also might slow the process
down. My best guess is you're going to need a much longer
Hello,
I need some input on a manual tissue processing protocol I have received.
(5 columns: reagents; tiny to large tissues...)
Reagents\embryoes\small tissue\medium tissue\large tissue
70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON
95% EtOH\30 min--1hr\1 hr\1hr\2hr
95% EtOH\30 min\30 min\1
