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> Subject: [Histonet] p2y12 cryo rat problem high background
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-Original Message-
From: Kooijman, E.J.M. (Esther) via Histonet
Sent: Thursday, November 15, 2018 5:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p2y12 cryo rat problem high background
**This is an External Email - Please DO NOT open attachments or click links
Hello all,
I am trying to stain cryo brain sections from the rat 7um but having a lot of
background. What am I doing wrong. Below the protocol is used and tried to
adjust…
1- Fix the tissue with cold acetone (-20 ⁰C) for 10 minutes
2- Let the slide dry for 30 minutes at room
