Re: Her2 by IHC
We reflex 1+ to FISH, too
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
___
Histonet mailing list
My lab does not have a written policy on this yet but are discussing it.
If the patient has a high grade tumor and HER2 IHC is 1+ (or even 0+), some
of our pathologists will reflex to FISH. The oncologists will request it
sometimes too, more often when ER and PR are both negative. We have found
Craig,
CLIA licensure gives your lab the authority to validate and use ASR's for
diagnostics. For something like her2 you will need to compare it to an existing
FDA approved antibody, run the statistically significant number of cases (range
of positive, and negatives) and show high
Yes, check the ASCO/CAP guidelines there are correlation rates for positives
and negatives.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: mw...@wakehealth.edu
To: histonet@lists.utsouthwestern.edu
Date: Thu, 22 Jan 2015 20:21:53 +
Subject: [Histonet] Her2 IHC validation
In
That is true.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is intended for the
We use Integrated Oncology-Lab Corp, 521 West 57th Street, 6th floor,
New York, NY 10019 800-447-5816
Josie Britton HT (ASCP) QIHC
Cheshire Medical Center
580 Court Street
Keene, NH 03431
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Thank you Josie this is very helpful.
Joelle Weaver MAOM, HTL (ASCP) QIHC
Subject: RE: [Histonet] HER2
Date: Wed, 14 May 2014 07:22:39 -0400
From: jcbrit...@cheshire-med.com
To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
We use Integrated Oncology-Lab Corp, 521 West
Thank you for the references,
I have found what I needed in this article:
Minimum Formalin Fixation Time for Consistent Estrogen Receptor
Immunohistochemical Staining of Invasive Breast Carcinoma. Goldstein NS, et.
al., Am J Clin Pathol 2003;120:86-92
Willem
My mistake: the web site is:
Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa
Verzonden: ma 10-12-2012 18:23
Aan: vtol...@cox.net; histonet@lists.utsouthwestern.edu
Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing
Val:
Acknowledging that you have a fixation problem is the fundamental
Subject: Re: [Histonet] Her2 Dual ISH and breast processing
Please go to http://www.histosearch.co/rene.html
There are 2 articles on the subject: one about the general fixation issue and
another about the minimum amount of NBF required to obtain complete fixation.
René J.
From: Hoekert, W.E.J
; vtol...@cox.net;
histonet@lists.utsouthwestern.edu
Sent: Thursday, December 13, 2012 7:35 AM
Subject: RE: [Histonet] Her2 Dual ISH and breast processing
I was wondering,is there any literature on this subject? i.e. the minimal
required fixation time of breast tissue in order to get reliable immuno
Val:
Acknowledging that you have a fixation problem is the fundamental step to
solving your staining problems.
You state that the slices have now a consistent thickness of between 2-3 mm and
that is great BUT what about the fixation time?
Thin slices is a first step but until you have the slices
Margaret
Dako has a bunch of info on Her2 staining in gastric cancer its in the
most recent Connection Volume 15 and they also have a companion document
that covers Her 2 staining and scoring for gastric cancer, just go to
the Dako website and you should be able to view these documents.
Liz
We do use the Ventana antibody for gastric cases. There is some difference
in reading the slide for the pathologist. I'll send you an article on it in
a seperate e-mail (so I can attach it).
Mark
On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret copp...@aruplab.comwrote:
Hello,
I am
@lists.utsouthwestern.edu
Subject: Re: [Histonet] Her2 Gastric cases
We do use the Ventana antibody for gastric cases. There is some
difference
in reading the slide for the pathologist. I'll send you an article on
it in
a seperate e-mail (so I can attach it).
Mark
On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret
02/03/2011 04:54 PM
To
Weems, Joyce jwe...@sjha.org, Paula Lucas plu...@biopath.org,
histonet@lists.utsouthwestern.edu
cc
Subject
RE: [Histonet] Her2 Fixation Requirement
Does anyone have issues with inking the specimen after it has been placed
in formalin?
-Original Message-
From
Lucas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement
We have the nurse document the time of removal on the requisition. The next
requisition update will have a spot for this. They have been very cooperative
and do a good job.
In addition, you must document
We receive our lumpectomies and total breasts fresh. They are immediately
put in 10 % formalin if no frozen is required and that time is noted on our
requisition. Our processing time for formalin and the fixation time is made
part of the final report. If we receive a breast biopsy specimen we
We have the nurse document the time of removal on the requisition. The next
requisition update will have a spot for this. They have been very cooperative
and do a good job.
In addition, you must document the cold ischmic time - that is the time from
removal until time in formalin. This is
What is the appropriate time it should be in formalin? Do you start your
time when it is initially put into formalin, or the start from the time it was
dissected?
Lori A Disher
Fawcett Memorial Hospital
Port Charlotte, FL 33952
lori.dis...@hcahealthcare.com
, 2011 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement
What is the appropriate time it should be in formalin? Do you start
your time when it is initially put into formalin, or the start from the
time it was dissected?
Lori A Disher
Fawcett
, February 02, 2011 14:16
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement
What is the appropriate time it should be in formalin? Do you start your
time when it is initially put into formalin, or the start from the time it was
dissected?
Lori A Disher
Fawcett
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, February 02, 2011 2:28 PM
To: lori.dis...@hcahealthcare.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement
Lori
We left it up to our pathologist. Initially we did the 25 slide validation,
our pathologist wasn't 100% convinced that he could read them accurately, so
we did another 25, plus all of the ones we sent out which already had FISH
ran. It ended up being almost 70 cases, and our pathologist was
Thank you all for help with the Her 2 policy. I was really stuck
writing the processing piece. I was able to finish the policy now
to get our docs to pass it.
THX again for the great help!
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
We've been getting these requests lately too. I've been wondering if we
need to monitor fixation time for these specimens, since Her2neu is a
possibility.
Mark Tarango
On Mon, Nov 2, 2009 at 9:44 AM, Maray Weirauch mweira...@crittenton.comwrote:
We have had some oncologists asking for HER2 by
Amy:
The question has the answer in itself. You have to do the comparisons in the
amount recommended between results in your lab vs. in other labs. That is what
a validation requires.
René J.
--- On Tue, 11/4/08, Amy Self [EMAIL PROTECTED] wrote:
From: Amy Self [EMAIL PROTECTED]
Subject:
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