Yes, you need to remove it. Hydrate your sections and place them in a 1% aq.
acetic acid solution (it will be a weak acid solution) and you will see how the
eosin fades and the hematoxylin becomes reddish. Keep them in the solution
until almost all the hematoxylin becomes less evident but it
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Subject: Re: [Histonet] IHC (DAB) after HE?
Yes, you need to remove it. Hydrate your sections and place them in a 1% aq.
acetic acid solution (it will be a weak acid solution
Of MARY T HODGES
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Subject: Re: [Histonet] IHC (DAB) after HE?
I have just ihc over
No. Re-hydrating the tissue will remove most (if not all) of the eosin and the
hematoxylin will serve as your counterstain (although you may need to
counterstain again at the end of you protocol). If you use antigen retrieval
for an unstained slide, you will have to use it on the HE-stained
