Colleen,
The antigen expression you are getting in the human tonsil may not be
expressed in the mouse femur, plus u have to make sure the ab u are using
cross reacts with mouse tissue, if it is anti human it may or may not react
in mouse, the ab data sheet should indicate species reactivity. The
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
koelli...@comcast.net
Sent: Thursday, August 15, 2013 10:17 PM
To: Colleen Forster
Cc: Histonet
Subject: Re: [Histonet] IHC after EDTA decalcifying
Coul
Could I ask exactly what you are looking for in mouse femur and human tonsil
the tonsil I assume is a control? For what? Whatever you are looking for is
obviously in human tonsil. Are you positive it is in murine femur. I used to
stain for 15-20 targets in EDTA decaled mouse femurs but my
Hi Colleen,
We use "ChelatorCal" from BBC Biochemical, which contains EDTA. It is very
gentle and retains antigenicity for IHC stain. We really like it.
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
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