Richard
We just started working on a IHC protocol for p21 we tried the antibody from
Dako, we looked at 1:25 and 1:50 at one hour RT incubation with pH9 retreival
on some colon CA blocks, stain is specific to the nuclei with no background so
far, but that's about all we have done, we were
Calbiochem p21 (1:100) and Cell Signaling for the p27 (1:250)
We use the Dako Flex Reagents on the autostainer. Low pH retrieval.
I have more detailed protocol if you need it.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
