We purchased a Universal Negative reagent from Ventana and then we use that in
a prep kit, totally eliminating the need for Mouse and Rabbit negatives and a
lot of extra space on the carousel.
Shannon Gower
Senior Histotechnologist HTL(ASCP)
All Children’s Hospital
St. Petersburg, FL
We run patient tissue as a negative and we use diluent as the reagent negative.
TH
Message: 7
Date: Fri, 17 Jul 2015 14:48:17 +
From: Abbott, Tanya tanyaabb...@catholichealth.net
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
Dawson; histonet
Subject: RE: [Histonet] IHC negative controls
Can anyone tell me if JHACO CLIA are deferring to CAP's judgment that a
negative control is not needed when utilizing a polymer detection?
I assume that this is the case, but I'd like to be sure.
Short answer:
Don't bet the farm
-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, November 05, 2012 12:56 PM
To: Morken, Timothy; 'Glen Dawson'; 'histonet'
Subject: RE: [Histonet] IHC negative controls
The thing is in my opinion that having the backing of CAP on this issue is a
good argument to make to CLIA
Ruegg; 'Glen Dawson'; 'histonet'
Subject: RE: [Histonet] IHC negative controls
Could be, but my point, maybe not clear in my answer, is that you should
check with your accrediting agency before an inspection and not assume that
they will follow what CAP has said it will do.
Tim
-Original
] IHC negative controls
Hey Glen,
Don't know the answer to your specific question but on a related issue,
our lab has decided to maintain running negative controls due to the
fact that the manufacturer's data sheets for our IHC detection kits and
a majority of our antibodies require using
an extensive
validation is adequate.
Good luck with your decision,
Linda
-Original Message-
From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org]
Sent: Monday, October 29, 2012 5:23 AM
To: Sebree Linda A; Glen Dawson; histonet
Subject: RE: [Histonet] IHC negative controls
Very good
Hey Glen,
Don't know the answer to your specific question but on a related issue, our lab
has decided to maintain running negative controls due to the fact that the
manufacturer's data sheets for our IHC detection kits and a majority of our
antibodies require using +/- controls. The company in
Can anyone tell me if JHACO CLIA are deferring to CAP's judgment that a
negative control is not needed when utilizing a polymer detection?
I assume that this is the case, but I'd like to be sure.
Short answer:
Don't bet the farm on it. Each enforces CLIA regulations but have different
