Tell the pathologist to stop fixing the smears. Let the smears air dry and
later you fix/stain them. Besides fixing with 2-propanol is not the way to fix.
If they also dismiss this procedure, then the will end without stained smears.
René J.
--- On Wed, 1/12/11, Fawn Bomar
We always used 95% alcohol to fix, but you're right the blood usually
comes off. Air dry then diff quik (or giemsa) stain works better for a
hema-pathologic stain. You could try Pen Fix (or an alcoholic formalin)
and this might help as opposed to the 95%. Why does the pathologist
need to see
Our procedure is as such: The FNA of the thyroid is place in 5mls of
plasmalyte ( an electrolyte solution). We lyse the red blood cells and
make cytospins.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805
I guess that you're staining these fix slides by Papanicolaou?, perhaps the
smears are too thick?,
we process daily different kind of smears and always prepare one fix and one
air dry smear for Pap and Giemsa staining and occasionaly we have an issue only
very muccous samples which can coming
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thyroid Smears
I guess that you're staining these fix slides by Papanicolaou?, perhaps the
smears are too thick?,
we process daily different kind of smears and always prepare one fix and one
air dry smear for Pap and Giemsa staining
KIRBIS'; fawn.bo...@halifaxregional.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thyroid Smears
Try using plus slides and make the smears very thin. Then it won't matter what
kind of fixative you are using everything will stick.
Sharon Davis-Devine, CT (ASCP)
Cytology
