[Histonet] RE: reagents for IHC

I agree with BioCare BioCare BioCare. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From:

RE: [Histonet] Re: from PLEASE HELP

I believe you are describing the procedure for Michel's Fixative Solution. Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Michelle MacVeigh-Aloni Sent: Mon 8/3/2009 7:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: from

[Histonet] ACELL compressed pig bladder

Hi to all, Our facility is experimenting with a matrix made from acellular, compressed pig bladder, as an alternative to collagen in the making of engineered skin for burn patients. The name of the material is Matristem Wound Sheet and Matristem Surgical Matrix. It is not very thick, but extremely

[Histonet] RE: reagents for IHC

Hi Everyone, I do appreciate for all suggestions I get form most of you! Have a nice week, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] golgi stain- please help

Dear Histonetters, I was performing a golgi stain on mouse brains and my treated animal group gave nice results but the untreated control group did not show any staining at all (it looks like the staining didn't work). Does anyone know / have an idea, if I could repeat the staining on these

Re: [Histonet] golgi stain- please help

Golgi staining and related methods are done on whole, fresh pieces of brain tissue, not on sections. There are some techniques for fixed specimens. Instructions can be found in books of neurohistological technique; older ones often have plenty of technical tips. A good start would be: Santini,

[Histonet] Brucella IHC

I'm posting this for a researcher. Does anyone out there do IHC testing for Brucella canis on fixed tissue? If so, please contact me directly. Thanks, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory

[Histonet] Golgi stain

Frauke, There are some published methods for doing Golgi on brain sections: Freund TF, Somogyi P. The section-Golgi impregnation procedure. 1. Description of the method and its combination with histochemistry after intracellular iontophoresis or retrograde transport of horseradish

[Histonet] Re: reagents for IHC

Are you using automated IHC-staining machinery, Naira? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: WindoPath

Chisti, We've used WindoPath for the last 8 years. Good AP system. Good archives, can make modifications to suit your institution, etc. The only negative that I can give you is: Stay far, FAR away from the 6.0 version. We are using it, but can't wait for the hospital to approve the budget for

[Histonet] RA Lamb paraffin

One of my colleagues has been using paraffin from Raymond A Lamb for embedding rodent brains. She loves it, but now that ThermoFisher has acquired RA Lamb this paraffin is being discontinued. Does anyone know of any other distributors that might still have some stock...or an equivalent substitute

[Histonet] Laboratory Openings in New York Area

Hello, Below are a few of the positions we are currently working on in NY. We are offering up to $1,000 for referrals if we place qualified candidates in these openings. These positions vary from Histology to Medical Technologist positions. Any help would be appreciated. I’ve listed the

[Histonet] Several new opportunities with Leica Microsystems

Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and

[Histonet] Paraffin Sections

Hi Histonetters, When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. Thanks for your suggestion and help.

Re: [Histonet] Paraffin Sections

Peri, Sounds like poor infiltration to me. What kind of tissue are we talking about, and what processing program did you use? Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854

[Histonet] (no subject)

Part-time job opening for an experienced HT or HTL in St. Augustine, Florida. It is a new lab for a group of gastroenterologists. I estimate it will require 4 to 6 hours per day. Very flexible hours; design your own schedule. Pay is commensurate with experience and local scale. Great working

[Histonet] Tissue Processor Advice

Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you

[Histonet] Eosin Bleeding

We very occasionally have a single slide out of a group of 100 or more where the eosin will bleed after coverslipping. Does anyone know if this is associated with a particular tissue type or treatment with Nair to soften keratin? Thanks, Tresa Veterinary Diagnostic Lab Bozeman, MT

[Histonet] Hi folks, looking for contact information for a lo...

Hi folks, looking for contact information for a long lost histotech colleague. Looking for a dear friend named Fran Lemons or Fran Walker. Last known to be working in Tennessee. Feel free to contact me with a private e-mail. geowei...@hotmail.com Thank you all, Georgia Weigel, HT(ASCP)

Re: [Histonet] Paraffin Sections

You running particularly small specimens like derm? You may need to decrease time in alcohol. Adding a little ammonia water to your ice bath and soaking after facing the block may help too. --Original Message-- From: Kathleen Roberts Sender: histonet-boun...@lists.utsouthwestern.edu To:

Re: [Histonet] Tissue Processor Advice

I recommend the new tissue tech. I use it in my lab and its reliable and intuitive. I strongly warn against TBS. I use one in my other lab and its been nothing but trouble. Its chemical storage is unreliable and leads to cross contamination. --Original Message-- From: Igor Deyneko

[Histonet] working with mouse liver...

Hi all, We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.) We use commercially made 10% NBF and tried fixing for

Re: [Histonet] working with mouse liver...

In my experience you are way over processing the tissue.  Drop your processing times to about 30 min per station.  That should be fine. Roger Moretz, Ph.D. (ret) - Original Message From: Michelle MacVeigh-Aloni macve...@usc.edu To: Histonet@lists.utsouthwestern.edu Sent: Tuesday,