[Histonet] Mysterious artifact on GI biopsies HE stain

Hi, We have been getting a similar staining artifact on some GI biopsies randomly while the rest GI biopsies look fine. Also on the affected GI biopsies there is often only 1 or 2 spots of the biopsy that are affected while the rest of the biopsy looks fine. The artifact is being

[Histonet] paraformaldayhde

In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Mysterious artifact on GI biopsies HE stain

I would check to see what medication the patient was on or if something was done during the procedure to affect the biopsies.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday,

[Histonet] anti 8 hydroxyguanosine

Hey guys was wondering if any of you used the anti 8 hydroxyguanosine to detect free radical induced DNA and RNa damage. which antibody is the best. we tried the one from abdserotec and it is a disaster. the background is just horrible. any idea ? 2010/4/26 Perry, Margaret

RE: [Histonet] Mysterious artifact on GI biopsies HE stain

It could be a hot biopsy? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 6:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on

[Histonet] Any Histology openings in San Diego area?

Hi Netters, I have a friend looking to relocate to San Diego area and is looking for a Histology position, she is certified HT. Any help would be greatly appreciated, thanks in advance!! Jill Jill Cox HT (ASCP) Arizona Dermatology 4232 E Cactus Rd Phoenix AZ 85032

[Histonet] (no subject)

Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ___ Histonet mailing list

[Histonet] Re: Two antibodies from the same host with tyramide

Adam, Thanks for the detailed protocol. I can't explain it either but I'll throw some ideas out there and maybe it'll stimulate discussion. Hopefully Chris vanderLoos will chime in. Have you tried doing the labeling in reverse, using the VE-Cadherin first and then blocking with the goat IgG,

RE: [Histonet] (no subject)

I highly recommend the Milestone microwave tissue processors. I have used their microwave tissues processors in two different locations and have found them to be very reliable, user friendly, reproducible and cost effective. They also have wonderful tech support. Kaye Ryan Histology Supervisor

RE: [Histonet] (no subject)

It really depends on your volume to be processed and what you are planning to process. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 10:10 AM To:

RE: [Histonet] (no subject)

I would look at this very carefully, especially in light of the new regulation that were just released by the CAP on ER and PR. But that is up to each individual lab Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ

[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?

This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out --Hadi or Rich I know they only list two PR clones one is Dako 1294-what is the other 312? Deb From: ih...@googlegroups.com

[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?

Did I understand correctly from Dr. Hammond in Florida that the ER ASCO/CAP guidelines extended the fixation time to 72 hours? Are they changing the Her2 guidelines to match the ER? If so, has that happened yet? I have people very anxious to stop having techs work on the weekends to comply with

[Histonet] histotech positions in San Diego area

Life is uncertain, but We may have an opening in the future. You may send resumes directly to this email address and I will forward them on to the appropriate people. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway

[Histonet] Fibrinogen Ab

Does anyone know a fibrinogen antibody that reacts in pig? Thank you in advance. Joel R. Israel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Two antibodies from the same host with tyramide

Adam, I've been doing some searching and the tyramide shouldn't shelter subsequent IHC reactions, so I don't think that's it. I found evidence of co-localization of mRNA ISH signal with tyramide enhancement and IHC staining without amplification in a publication from J Histochem Cytochem. I'm

[Histonet] Ventana EMA antibody

Histofriends, Has anyone had success staining an ependymoma with Ventanas EMA antibody? My meningiomas stain nicely. I have a case that I've stained with Dakos EMA on an Autostainer, and it shows the expected spotty pattern. Anyone out there have a successful protocol? Happy Monday, Angie

[Histonet] Re: paraformaldehyde

Margaret, good luck getting 37 grams of powdered PFA to go into solution. Even making a 10% solution is difficult, usually requiring heat (60 degrees C) and/or sodium hydroxide to get it into solution. Once you do, it's going to want to repolymerize and you'll end up with a real mess. You'd do

RE: [Histonet] Re: paraformaldehyde

I ditto Teri's suggestion. In a recent email to you, I mentioned the dangers of using powdered paraformaldehyde. I have since stopped using it. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] Gina Rodriguez is out of the office.

I will be out of the office starting 04/26/2010 and will not return until 05/03/2010. I will respond to your message when I return. __ This email has been scanned by the MessageLabs Email Security System. For more information

[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?

Thanks Michael I saw table 10 -but on page 16 they specifically only list clone 1294 and 312 (which is whose?) -they really don't clearly say all clones for PR listed in table 10. They also only refer to these two again on the shortened ASCO color plate handout Additional information in re:

RE: [Histonet] ER clone 1D5 or SP1 ?

Hi A large number of studies have been performed which show that 6F11 will stain a number of tumours that 1D5 does not and I know a number of labs who have moved away from 1D5 because of this. Google 1D5 and 6F11 and you will find the articles. regards Tony Tony Reilly B.Sc.

[Histonet] Bone Marrow Clots - falling off slides

Hi Histonetters! We have started to have an issue with our bone marrow clots falling off the slides. We are using plus slides, making sure they drain well (just like our other slides), but when we stain them routinely, we are getting a fair amount of tissue coming off the slides. It has been

RE: [Histonet] Bone Marrow Clots - falling off slides

recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols From: histot...@imagesbyhopper.com To: histonet@lists.utsouthwestern.edu Date: Mon, 26 Apr 2010 23:26:43 -0400 Subject: