How disgusting to hear about cheating. I recall that someone was supposed to
sign off as a witness that the applicant had done the work themselves. I spent
months acquiring tissue, processing, embedding, cutting and staining a set of
blocks and slides and was rewarded with a high score for
Thank you Joe!!
Nancy Heath, HT(ASCP)
-Original Message-
From: Galbraith, Joe [mailto:joseph-galbra...@uiowa.edu]
Sent: Thursday, August 25, 2011 2:07 PM
To: Bea DeBrosse-Serra; 'Jennifer MacDonald'; Heath, Nancy L.
Cc: Histonet Listserv (E-mail);
Ditto Joe! I did it twice! Once for HT, then again for HTL!!!
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
From: Galbraith, Joe
Man did that bring back some memories. Bill and I worked together in the same
Navy lab and he is correct if a block was embedded wrong you would get it in
either the back of the head or between the shoulders. They stopped throwing
them at me the day I throw the block back at the microtomist and
Back to the question at hand,
You will need to engage the pathologists to provide information as to the
correct way to embed the skin specimens. If you have a dermatopathologist in
your practice, that person will need to provide the information about how
he/she dissects it, what he/she wants,
Starting with the gross dissection manual, pathologist input is excellent. I
always assume that education is key! Thanks for points, good comment to this
discussion
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: Johnson Teri t...@stowers.org
Date: Thu, 25 Aug 2011
Lucky me.
Being a MT in Austria I've learned also histotechniqe during education. ;)
So no discussions like this. But in reality people working in histologic or
chemical labs are of different species.
I think managing and technical supervising are different skills. In small
teams both is in close
Hello Histonetters,
Does anyone know if there is a rule as to MSDS binders having to be in yellow
and black bold lettering? Do we even still need to have hard copy if we have
access to msds.com on desk top? I am in Ca and will be CLIA licensed.. Thank
you in advance!!
Jill Cox, HT ASCP
Not certain about CLIA, but CAP only requires immediate available access to the
MSDS's by any personal using chemicals. I interpret that as digital copies ok,
as long as everyone has access. I have passed three inspections with digital
MSDS only.
Will Chappell
Sent from my iPhone
On Aug 25,
Thank you for clarifying the essential element at hand. I have learned to
appreciate the different skill sets indeed. Lucky you for having training in
all.
Joelle
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: Gudrun Lang gu.l...@gmx.at
Date: Thu, 25 Aug 2011 18:53:09
In practicality you would turn to your online version first. What
happens if the computer system/network is down. Seems like it would be
prudent to have a hard copy available.
Victor
Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of
Histo Folks- please share what you are using to soak instruments and wipe
down counters.
What we were using is no longer available.
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
I thought it might be good to study and try for the HTL (already have school
for HT and am certified) but it looks like the time limits prohibit me from
trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly
buy the books and study. Cannot get into a lab here in NC
There must be some way for the Fire Department to be able to access MSDS. As
Victor rightly points out, if your computer system is down, that option no
longer exists. It is not only prudent, it is mandatory for the Fire Service to
be able to look up any chemical within your facility.
If you
Our Fire Marshalls even require we have a big red sign pointing to where the
hard copies are located. They don't need to hunt for it in case of an event.
Shirley
From: histonet-boun...@lists.utsouthwestern.edu
We also use Lysol I.C.
Caula Gill(HT)ASCP
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree
Linda A
Sent: Thursday, August 25, 2011 2:07 PM
To: barbara.cr...@lpnt.net;
We do have hard copy of our MSDS, my question was mainly if we are able to use
same binder type as our procedure manuals for our MSDS or does it have to be in
a special color binder like bright bold yellow/black?
Jill Cox, HT ASCP
___
Histonet
Tom, (and all those following this thread)
And we got that ;) perfection.
(Warning, long rant ahead... Rene's might have been longer, but he
spread it out over a number of posts)
(Hi Rene, I enjoyed your rant.)
My biggest barrier in assimilating into the civilian workforce was
that perfection
I would say if your computer system is down, then your electricity is
probably out so I would hightail it out of there. Or ask yourself, why am I
working with hazardous chemicals in the dark?
Our EHS allows digital MSDS as well, but they say you could always call them
if necessary. So maybe
Actually now that I think about it, it would be faster to have a digital
system, as you could search the MSDS file with the find option to get
exactly what you needed. Also the most updated copies would be online
always and you could google for them.
And you would haven't to remember the actual
Linda,
Where do you get your Lysol IC?
Rae Staskiewicz
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Thursday, August 25, 2011 1:07 PM
To: barbara.cr...@lpnt.net;
We get ours from Lab Safety Supply.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae
Staskiewicz
Sent: Thursday, August 25, 2011 2:54 PM
To: 'Sebree Linda A'; barbara.cr...@lpnt.net;
I will be out of the office starting 08/25/2011 and will not return until
08/29/2011.
Note: For Cytology issues, please call Molly at 8-421-5487, Eric at
8-421-5405, Barbara at 8-421-5033, or Wanda 8-421-5426 For Histology
issues, please call Mario at 8-421-4961, general histology lab
Greetings all,
I contacted CAP just before my on-site inspection last year. I was told that
all validation studies had to be on file until the antibody/ special stain/
or equipment was no longer used. Sad to say. Now, I understand for 2012, CAP
has added another lab specific checklist. My
Hi all
We would like to prepare cryosections of adult zebrafish (3 - 6 months old).
Fixation is not allowed. Is anyone of you familiar with the preparation of
cryosections (including fish bones, and spinal cord)?
I assume that especially the spinal cord will cause problems.
Joost Bruijntjes
Hi Tom,
Thank you for your kind words. I am off the bench almost completely. I can
work in the gross room in a pinch and my counting skills are excellent, so I
can always file slides and block if an emergency arises:) I occasionally cover
a bench in Chemistry as well, but my staff is all
This is exactly why the powers that be should have NEVER gotten rid of the
practical portion of the HT/HTL board certification!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent:
This is exactly why the powers that be should have NEVER gotten rid of
the practical portion of the HT/HTL board certification!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
D'Attilio, Shelley
Sent:
We use a black and decker rice steamer fairly routinely. It has the ability to
steam for a total of 75 minutes, although it takes a good 15 of them to get up
to temperature. I like it becasue the temperature is nice and even and it is
very easy to do.
1. add water to the bottom of the
Dear Shelly,
I would work for you without reserve.
I have managed both cytometry and Electron Microscopy successfully, and I
cannot do either technique. However, I understand enough about it to make sound
decisions and empower my people enough that it works well. It is possible to do
well if
Well said.
Johnson, Teri t...@stowers.org
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/25/2011 07:39 AM
To
Histonet histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] Re: Embedding process improvement and competency assessment
Dear Shelly,
I would work for you
I don't normally do frozens on tissue that has some pathogen but have been
asked to cut slides on mouse tissue that has been infected with cowpox.
I can take that precautions while doing the frozens but what can I use to
disinfect the cryostat after the project is complete?
Andi
I fail to see the correlation of a non HT person supervising the Histology
lab and the lack of a practical exam for HT/HTL staff. One of the issues
that Shelley brought up was the staff lost or did not develop their
embedding skills. Submission of a practical exam is not proof of highly
One of the new CAP questions is ANP.22976 ER/PgR validation.
If the laboratory performs immunohistochemistry for estrogen receptor and/or
progesterone receptor as a prognostic/predictive marker on breast carcinoma,
the laboratory has documented appropriate validation for the assays. In the
Please respond to all. I would like the information also.
Thank you,
Carol
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Thursday, August 25, 2011 12:01 PM
To:
I have produced a very detailed guide for my techs. A little long, it is about
8 pages including diagrams pertaining mostly to derms. I would be glad to
forward to anyone interested.
I would also like to share a book. I met the author and she autographed my
book at a North Carolina meeting
I hope you're correct.
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
-Original Message-
From: Martha Ward [mailto:mw...@wakehealth.edu]
Sent: Thursday, August 25, 2011 11:30 AM
To: Carol Bryant; Vickroy, Jim;
Regardless of wether there were 8 blocks or eighteen blocks taking the
practical taught me to be precise with all of the hands on aspects of
Histology. Shame on the older techs from the practical days of not
keeping on top of their game with embedding. My comment was geared more
towards the
I think it may depend on the inspector. We had something similar happen
in Cytology during inspection. They had no validation records for their
Thin Prep processing, which they had been doing for years. They were
required to validate and provide documentation to CAP.
Laurie Colbert
That is unfortunate that people will resort to dishonesty, only cheating
themselves in my humble opinion... According to NAACLS, since the
discontinuation of the practical component of the HT exam( per Zoe) it is the
responsibility of the program directors to require practical blocks and
I might be wrong here but I thought that each time that you brought in new
testing, you had to validate it at that time, then had to keep those records
for the life of the test, plus a couple of years.
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
-Original
I heard of a lot of cheating as well. People paid others to do the blocks and
staining. How good does it do? In the end, these people are cheating
themselves. Very sad!
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
This point is well taken- I agree that our experience and knowledge often goes
unrecognized. In my humble opinion , it takes all components, theory knowledge,
extended practice and forthcoming technical mastery... some of this can come
with academic study, some comes with actual doing . No
I also like this reference- I like the quote. Quality embedding is key to me to
the ultimate production for high quality, and sometimes discounted and
overlooked in that regard.
Joelle Weaver MAOM, BA, (HTL) ASCP
Date: Thu, 25 Aug 2011 09:29:17 -0700
From: kdboydhi...@yahoo.com
To:
Yes, but I don't think this has always been the case, or at least it
hasn't been strictly enforced. With all of the new specific CAP
guidelines regarding validation, it is not only a requirement now, but
the expectations/requirements are more clearly spelled out. When I
started here in 2000, we
It is my understanding that one should also revalidate when you switch
antibodies, detection kits or instruments/methodology.
As far as validating against another source, you should validate against
someone doing the same methology, same antibody, and same detection kit.
Thanks
Debbie
I would love to have a copy of your embedding guidelines.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
My materials management department told me that AMPHYL has been discontinued.
Ventana recommends that we clean/decontaminate the Benchmark and the Ultras
with AMPHYL.
Has anyone else ran across this? Has AMPHYL really been discontinued?
Is there a substitute we can use.
Thanks everyone!
Just to throw a somewhat funny situation into the mix. I learned my
embedding skills in the Navy. The basic method for learning all tasks
was 1. explain it, 2. demonstrate it, 3. do it. Each microtomist was
responsible for taking a good look at the block before cutting it. If
the embedding was not
All:
It is my understanding that you should retain validation records for any
testing that is still being done regardless of how long ago that validation may
have occurred. In general, you need to be able to show how you traced and
compared the current test to some previously validated test
We use Lysol I. C.; haven't be able to get Amphyl for years.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
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