Overnight processing is usually optimal for average size tissue. Larger tissue
may not get optimal processing and biopsies may be a little over processed. It
is the nature of tissue processing in a general lab that does not have several
processors with different programs. A solution is to
Dear all,
We are trying to establish an anti-GFP staining with rabbit polyclonal A11122
from Invitrogen.
We are using formalin fixed, paraffin embedded mouse tissue and want to
distinguish between a GFP transgenic mouse strain and a wt mouse.
The problem is, we get a strong background in the wt,
Hi NSH'ers - Program #22 of HistoTALK www.HistoTALK.com has a great interview
with our Executive Director, Carrie Diamond and Brenda Royce our Membership
Manager. Both ladies share with us a huge amount of information and ideas about
our special day - Histotechnology Professionals Day on March
Wish I could hit the like button for this email ;)
Message: 10
Date: Fri, 24 Feb 2012 14:01:09 -0800
From: Davide Costanzo pathloc...@gmail.com
Subject: RE: [Histonet] Grossing techs
To: Bruce Gapinski bgapin...@pathgroup.com,
histonet@lists.utsouthwestern.edu
Hey Chicagoland Histoneters,
We (Tech One Biomedical) are in the process of updating our fliers and
catalog. The designer and photographer were hoping to some shots of a
real lab. If you are willing to let us do it, please let me know. We
will do our best to stay out of the way of your work
I use this exact antibody routinely at 1:1000.
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
From: Marina Pils
Good Morning Histonetters
I am looking to add EGFR to our antibody list. What clone is everyone using
out there in Histoland? Any info would be greatly appreciated. Thanks in
advance.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 Ext. 232
e-mail
Anybody using anti-nFAT antibodies in mouse tissues or tcells? If so, could you
recomend a particular antibody?
Thanks!
Mike
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I'm venturing a few guesses here, and would welcome others to do the same. My
guess is using acetone might dissolve/soften the plastic from the tape but not
remove the adhesive from the top of the tissue section. In thinking about
removing adhesive from surfaces where it is unwanted, you
Soak the slide in acetone for 10-15 minutes; it turns to something that
looks like a slice of Jell-O and slithers down the slide, leaving cells
and stain and structure in perfect order. I usually dip the slide in
xylene a couple times to clear the acetone and re-coverslip it. Works
like a charm.
Is there an antibody out there that will stain for fibrin on FFPE mouse
tissues; been searching but haven't found one. Does everyone just do a special
stain when looking for fibrin?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
___
Histonet
Hi all,
I'm wondering if anyone knows of any per diem histology jobs in St.
Pete area. I am retiring from my current job and will be spending
several(winter) months in St. Pete.
Would I need a FL license to work per diem?
Thanks for any info you can provide.
Nancy Rutledge
Just a note on coverslipping tape - I love it - this is the first place in 40+
years I've used it, and it's good stuff. HOWEVER, I noticed a brown
cornflaking artefact on some tissues when I first arrived in this job - and we
fixed it. If the last dehydrating step (100%) ethanol has the
Greetings all,
My response was to the post sent by Merissa which states:
Dear All,
I am using clear packing tape on mma embedded rabbit femurs with implants.
The reasoning for the use of tape is that the implant crumbles when being cut
without the tape. I have switched to a new tape that
Bonjour,
Je suis absent du laboratoire jusqu'au lundi 05 mars 2012. Je vous répondrai le
plus rapidement possible.
Eric Tambutté
Thank you for your mail. I will be out of office till March 05th 2012.
I will respond to your e-mail as soon as possible.
Thank you for your understanding.
Best
We are trouble-shooting cryosectioning mouse lung tissue. Often the lung
section tears or breaks apart during sectioning. In the past, if the lung
section is proving difficult to section, we take the OCT-embedded tissue and
re-embed it back into OCT (basically put fresh OCT into the original
Well you can always run your lab in the most green way possible using recyling
and reuse management of all materials in the lab. In Md there are a few state
labs with coveted positions that only open up through the death or retirement
of a tech because they are so rewarding. The closest thing I
As long as you don't need to use them for RNA analysis, the easiest thing to do
is just rub your finger (without glove) across the block (very briefly) and
then take the section. This will probably do the trick and hydrate it just
enough to make a difference.
Kim
Kim Merriam, MA,
Also, make sure that the block sits in the cryostat for a while to acclimate to
the temperature. The cutting temp for lung (correct me if I'm wrong here) is
probably about -20; the block should be the same temperature as the cryostat.
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
Hello Histonetters,
Could someone please advise me on established guidelines for changing the
alcohol on the processor. We process a very small amount of blocks weekly on
our ASP300S. Approximately 20 blocks per week. We have decided to use a
hydrometer to test our 70% thru 100% alcohols,
Hi Kim,
I am working on a project just like this right now and we are using
Fibrinogen (AbCam ab34269) and PTAH. Drop me a line if you have any
questions.
Amos
On Mon, Feb 27, 2012 at 1:00 PM,
histonet-requ...@lists.utsouthwestern.eduwrote:
Message: 20
Date: Mon, 27 Feb 2012 09:34:08
Marina,
I agree with Paula Pierce, have used the same anti- GFP antibody on some
projects but at 1:500-1:1000 area and with a much longer 8-15 minute retrieval
in citrate. Also, I know you know to be aware of how much the mice are
transfected . In my transfected mice (NOT GFP and in a
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