[Histonet] Nuclear bubbling

Traditionally we regard nuclear bubbling as incomplete fixation however I'm not so sure that nuclear bubbling can't be caused by additional processing problems. This morning I have some GI biopsies that fixed for nearly 18 hrs that have a large amount of nuclear bubbling. We run the

[Histonet] Histology Supervisor Job in North Carolina

Happy Friday Histoland, I am looking for a qualified Histotech to fill a 2nd Shift Histology Supervisor role in North Carolina. Please contact me for more details. Looking for a Tech who is experienced in grossing and has some supervisory/lead/senior experience. Please reach out to me for more

[Histonet] Training ???

Can anyone share with me any worksheets you use to evaluate new trainees. Embedding, cutting, special staining things like that. How you set target goals? Thanks in advance, Happy Friday. Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601

Re: [Histonet] Tissue processing question

We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but

[Histonet] Tissue processing question

Hello all, I was wondering what everyone uses to secure biopsy and scant tissues through processing. Also what would you recommend placing breast cores in for processing. Having an argument with grossing staff and pathologist about whether to use sponges, tissue paper, or something else. Looking

Re: [Histonet] Nuclear bubbling

"Nuclear bubbling", manifested as round unstained areas in the nucleus, is caused by incomplete dehydration of the section before staining. There is a review on the subject that I cannot find at this moment.René On Friday, January 29, 2016 10:42 AM, "Vickroy, James via Histonet"

Re: [Histonet] Sponges for processing biopsies

Sponges are available in different qualities. We use very soft ones, that don't hurt the tissue. I know, there are also very hard materials on the market, that may render holes in the underfixed biopsies. Gudrun -Ursprüngliche Nachricht- Von: Lester Raff MD via Histonet

Re: [Histonet] Nuclear bubbling artifact

I've read about a group, that observed living cells during the fixation-process. They saw bubbling in the first period of contact and penetration of formaldehyde. After a certain time the bubbles disappeared again. Along this observation for me bubbles are a sign of too short fixation. Gudrun

Re: [Histonet] Nuclear bubbling artifact

Hi James, Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial cells, and GI biopsies are among those samples that are particularly susceptible to it. It has been linked to inadequate fixation and also to heating of slides prior to staining without complete air-drying

Re: [Histonet] Tissue processing question

Histoscreen cassettes will work as well. Generally the cassette options are expensive and may not work in all cassette printers, if you are using one. http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscreen-cassettes.html Ultimately, get samples of whatever you like to

Re: [Histonet] Tissue processing question

I really like this type: https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584 (although I buy them from mastertech, but they seem to have dissapeared from their website) They are great for both large tissues, and also biopsies. A

Re: [Histonet] Tissue processing question

Not advertising but I do a lot of research on tiny pieces of tissue and have found the perfect cassette from Cancer Diagnostics. It is the Vortex corner-less ones seen here.

[Histonet] Sponges for processing biopsies

WE use the biopsy sponges, THOROUGHLY soaked in formalin. They are easy to use and not time consuming either for the grosser or the histologist. About 98% of our volume is prostate biopsies, and we do not see the compression artifact Rene references. Unrelated blog

Re: [Histonet] Tissue processing question

LOL, we used cigarette paper worked fine. Now we use screen cassettes or HistoGel processing if super tiny. Michael Ann Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/29/16, 11:41 AM, "Walter Benton via Histonet" wrote:

Re: [Histonet] Tissue processing question

We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but

Re: [Histonet] Tissue processing question

Sponges can cause a compression artifact leaving some sort of "imprint" on the surface of the biopsy, especially kidney and prostate Bx.I my experience tissue paper is the best option. If you are having difficulties with the wrapping, you can use "tea bags".René  On Friday, January 29,

[Histonet] Clearance Angle on Microtome Blade

Good afternoon, Can someone touch base with me regarding the clearance angle of the microtome blade? How would you describe a blade clearance angle that is too great or too slight, and what microtomy problems are you likely to see with each? There seems to be conflicting information out there