[Histonet] Help with liver/heart tissues

Good afternoon, I am having a few issues getting nice morphology from both liver and heart tissues. I currently work only with CNS tissues (brain, spinal cord, DRG, etc.) but recently, our research team have become interested in immunostaining some peripheral tissues, including the heart and

[Histonet] Spill kits

Do spill kits need to be out where anyone can see them, or can I put it in a cupboard with a sign on the door? I need to move mine and there's very little room. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Over-processing of brain tissue

Good morning everyone: I seem to be having problems with over-processing of brain tissue. I use a VIP6 processor. I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol. Then half-hour each of three changes of absolute isopropanol. Then half hour each of three changes of xylene. Then

[Histonet] PAX 2

Good Morning- can anyone give me a recommendation for a predilute PAX2 antibody? The one I was using has been discontinued and the replacement product does not seem to perform very well. I will be using it on the Bond III. Thanks in advance! Jeff Robinson, Senior Histotechnologist, Sierra

Re: [Histonet] Spill kits

What you need to do is to communicate to everybody where the kits are, and place them where it is more convenient for you. Once everybody knows the location, a good sign is always a plus.René On Tuesday, June 14, 2016 10:15 AM, Anne Murvosh via Histonet

Re: [Histonet] Over-processing of brain tissue

It seems to me you are processing too much unless the slices are 3mm thick or more.I suggest you to cut the dehydration to 45 minutes (the sequence seems OK)Reduce the pure 2-propanol to just 2 changes (30 min is OK)Add 1 change of a mixture 1:1 of 2-propanol and xylene + 2 xylene stepsthen to

Re: [Histonet] Spill kits

We keep our spill kits in unobstructed cabinets with bright yellow signage on the wall above. We have no problem getting to them as needed, and have never had an issue in many, many CAP inspections. -- -- Latest post:

[Histonet] (no subject)

UT Path located in the medical center (Houston) is currently looking for a Medical Technologist that is able to work on an as needed basis, hours are negotiable. Duties include, but not limited to performing molecular testing using the Hologic Panther system, this could lead to a full time

[Histonet] Tissue controls

Hi Everyone, I am trying to help out a coworker with a survey on types of tissue and controls needed for both clinical and research labs. She has a short deadline by next Monday. She if in charge of the OriGene Technologies tissue division. If you could take a few minutes to fill in the survey

[Histonet] Cytology specimens

I would like to poll the Histo group concerning cytology specimens. The discussion has come up about when to discard a fluid after processing, and fluids that have no orders. Also, because we are a contracted service for the hospital, and are not connected to their LIS. Cytology's that are ordered

[Histonet] Several New Histology Career Opportunities!!

Hello Histonet, Our exclusively retained client has opened several new opportunities across the U.S., and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in cancer diagnostics and we are looking for