[Histonet] comparison between tissue processors

2019-02-19 Thread Julio Benavides Silván via Histonet
Hello everybody, we are buying a new tissue processor for the lab and, at the moment, we are considering these three models: Thermo Excelsior AS Leica HistoCore PEARL SLL MTM I/II I would very much appreciate your thoughts/experience regarding any of these models. We are a small research

Re: [Histonet] Coverslipper

2019-02-19 Thread Margaryan, Naira via Histonet
Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 just for $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the

[Histonet] FISH question

2019-02-19 Thread Gudrun Lang via Histonet
Dear histonetters! I have difficulties with my FISH preparation on FFPET. I struggle with massive background. It looks like a thick fluorescent film. The signals can't be seen because of the background. Even the nuclei are hard to see. The background is within the tissue but also surrounds it.

[Histonet] Best Interview Question EVER!! How would YOU answer it?

2019-02-19 Thread Pam Barker via Histonet
Dear Histonetters, I hope you are having a great week! Coming soon to an interview near you… Have you seen Facebook’s Million Dollar interview question? Here it is… “On your very best day at work - the day you come home and think you have the best job in the world -what did you do that day?”

Re: [Histonet] FISH question

2019-02-19 Thread Gudrun Lang via Histonet
Thanks for your advice. I use adhesive slides from Leica. Years ago we had Superfrosts of another brand (maybe Thermo) and I can't remember similar issues. Can you recommend a special brand for FISH? Have you ever tried to do FISH on a slide without adhesion? Gudrun -Ursprüngliche

Re: [Histonet] FISH question

2019-02-19 Thread Mark Tarango via Histonet
Hi Gudrun, Are you sure you have digested long enough with pepsin? If the tissue is not well digested you will see background. We use sodium thiocyanate for pretreatment reagent, not citric buffer. These are my first thoughts. Mark On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet <

[Histonet] job opening

2019-02-19 Thread Lauren Sweeney via Histonet
Hello Histonet, There is a job opening for a lab manager position at the University of Georgia. This is a small research lab in the College of Veterinary Medicine. There would be 2 techs, including yourself, and 2 pathologists. Since this is a state government job, the salary does not compete