Hi Igor. I am not sure of specific topics for 2009 but you should look at
the websites of ASCP, NSH, and TNT (teleconference network of TX). All of
these entities sponsor teleconferences. NSH will be in Birmingham, AL 2009
there are always great seminars there.
Patti Loykasek
Dear
Sarah:
Histoclear, also known as Histolene is a d-Limonene based xylene substitute and
there are 13 opinions about it in HistoNet that I will try to summarize for you:
1-not as good as Xylene to dewax before IHC
2-has a strong odor
3-hardens brain, liver and spleen
4-not good for cover slipping
Hi all,
a friend recently got a job in a veterinary histo and has some troubles in
cutting. He tells about paraffinblocks, where the tissue is swelling out
while warming like a small hill. I referred to the possibilty of taking up
humidity that causes swelling.
Do you know other causes for
Is he saying that he embeds processed tissue and removes the chilled
paraffin block from the base mold that it then swells up in the
paraffin? Before it is trimmed and placed on ice?
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
[EMAIL PROTECTED]
-Original
The major problem in a veterinary histo lab is generally 1 processor to process
all types of tissues and species. The lab I can from had this problem; you can
only optimize the schedule if you limit your tissues.
Some are under, some are over; and a small percentage are just right.
Rosa
Hi Gudrun,
Is your friend soaking blocks in ice water before cutting? This is
common in animal histology and over soaking before cutting will cause
swelling. Different tissue types will swell more quickly than others,
but over soaking can eventually affect all tissue.
Thomas Jasper HT (ASCP)
Kimberly, you asked:
Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block
Can someone break this down for me in simple instructions?
And
Its 1.1mg/ml
Another question: they are asking that I add glycerol to the antibody to
prevent freezing. Wont that change my
This is probably a really obvious question, but when doing Okajima's
stain for hemoglobin, is a blood smear an adequate control?
Thanks for any advice.
This communication is intended solely for the use of the addressee and may
contain information that is legally privileged, confidential or
Does anyone happen to have a good used tissue processor for sale? I'm also
interested in an embedding center and microtome.
Thanks
Mark
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Hello all,
I am looking for an anti-rat Sca-1 (Ly6A/E) antibody. If you know of one, I
would be so greatful for the information. So far I have found lots of rat
anti-mouse Sca-1. I have done mouse on mouse staining, but how do you do rat
on rat staining? Any information would be helpful.
I have a question for maybe some of the plant histotechs out there. How can
you best section cotton fibers? And yes I may be doing this soon.
Thanks so very much,
Cathy
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I'll be following this thread very closely haha
On 10/1/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote:
I have a question for maybe some of the plant histotechs out there. How
can you best section cotton fibers? And yes I may be doing this soon.
Thanks so very much,
Cathy
Peggy Wenk asked three days ago for information about radioactive
brachytherapy seeds in prostate tissue received in surgical
pathology.
I have seen one such case, about four years ago. I put the seeds in
a flatbed scanner and got a good clear picture of them, if you want
it.
Three radioisotopes
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