[Histonet] RE: reagents for IHC
I agree with BioCare BioCare BioCare. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Monday, August 03, 2009 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: from PLEASE HELP
I believe you are describing the procedure for Michel's Fixative Solution. Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Michelle MacVeigh-Aloni Sent: Mon 8/3/2009 7:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: from PLEASE HELP Hi Delia, Some time ago I used to work in a lab where I had to cut skin punch biopsies. They used to come floating in a special preservative. Unfortunately I don't remember its name, but then I had to wash them in a special wash solution for a minute. The wash solution was made by Zeus Scientific and was just citrate buffer. I am not familiar with the Michael's fixative and don't know do you have to wash after fixing. The trick was to pat dry the biopsies with Kimwipes and immerse them right away in the OCT. Then let the biopsy sit there for few minutes. You can even move the tissue in the OCT from time to time and then transfer it to a new mold with fresh OCT. Freeze in liquid Nitrogen. This way, somehow the OCT starts infiltrating the tissue and the tissue cuts together with the OCT. I used to cut it at 5 microns. Hope that this will work for you too Michelle Research Specialist USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ACELL compressed pig bladder
Hi to all, Our facility is experimenting with a matrix made from acellular, compressed pig bladder, as an alternative to collagen in the making of engineered skin for burn patients. The name of the material is Matristem Wound Sheet and Matristem Surgical Matrix. It is not very thick, but extremely condensed and somewhat dense, which is the total opposite of the bovine collagen we have been using. During rehydration it was not very hydrophilic, which makes me wonder about my processing times. Has anyone ever worked with this product? If so should I shorten/lengthen processing times? How does it cut? Any information would be greatly appreciated. Thanks, in advance. Deanna Leslie ASCP HT Shriners Hospital for Children Cincinnati, OH 45229 513-872-6388 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: reagents for IHC
Hi Everyone, I do appreciate for all suggestions I get form most of you! Have a nice week, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] golgi stain- please help
Dear Histonetters, I was performing a golgi stain on mouse brains and my treated animal group gave nice results but the untreated control group did not show any staining at all (it looks like the staining didn't work). Does anyone know / have an idea, if I could repeat the staining on these vibratome sections? I read something about deimpregnation of golgi stained slides, but found no protocol and I'm not sure if I can perform another fresh golgi stain with these slides. Any suggestion is welcome!!! Thanks to all of you, Frauke ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] golgi stain- please help
Golgi staining and related methods are done on whole, fresh pieces of brain tissue, not on sections. There are some techniques for fixed specimens. Instructions can be found in books of neurohistological technique; older ones often have plenty of technical tips. A good start would be: Santini, M., ed. (1975). Golgi Centennial Symposium: Perspectives in Neurobiology. New York: Raven Press. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Dr. Frauke Neff ne...@staff.uni-marburg.de Date: Tuesday, August 4, 2009 11:52 Subject: [Histonet] golgi stain- please help To: histonet@lists.utsouthwestern.edu Dear Histonetters, I was performing a golgi stain on mouse brains and my treated animal group gave nice results but the untreated control group did not show any staining at all (it looks like the staining didn't work). Does anyone know / have an idea, if I could repeat the staining on these vibratome sections? I read something about deimpregnation of golgi stained slides, but found no protocol and I'm not sure if I can perform another fresh golgi stain with these slides. Any suggestion is welcome!!! Thanks to all of you, Frauke ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Brucella IHC
I'm posting this for a researcher. Does anyone out there do IHC testing for Brucella canis on fixed tissue? If so, please contact me directly. Thanks, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Golgi stain
Frauke, There are some published methods for doing Golgi on brain sections: Freund TF, Somogyi P. The section-Golgi impregnation procedure. 1. Description of the method and its combination with histochemistry after intracellular iontophoresis or retrograde transport of horseradish peroxidase. Neuroscience. 1983 Jul;9(3):463-74. Somogyi P, Freund TF, Wu JY, Smith AD. The section-Golgi impregnation procedure. 2. Immunocytochemical demonstration of glutamate decarboxylase in Golgi-impregnated neurons and in their afferent synaptic boutons in the visual cortex of the cat. Neuroscience. 1983 Jul;9(3):475-90. Bolam JP, Ingham CA, Smith AD. The section-Golgi-impregnation procedure--3. Combination of Golgi-impregnation with enzyme histochemistry and electron microscopy to characterize acetylcholinesterase-containing neurons in the rat neostriatum. Neuroscience. 1984 Jul;12(3):687-709. Gabbott PL, Somogyi J. The 'single' section Golgi-impregnation procedure: methodological description. J Neurosci Methods. 1984 Sep;11(4):221-30. Harris KM, Cruce WL, Greenough WT, Teyler TJ. A Golgi impregnation technique for thin brain slices maintained in vitro. J Neurosci Methods. 1980 Aug;2(4):363-71. Spacek J. Dynamics of the Golgi method: a time-lapse study of the early stages of impregnation in single sections. J Neurocytol. 1989 Feb;18(1):27-38. Moss TL, Whetsell WO. Techniques for thick-section Golgi impregnation of formalin-fixed brain tissue. Methods Mol Biol. 2004;277:277-85. I don't remember if any of these discuss re-staining, but if you don't have any silver chromate crystals in the specimens it probably can't hurt to return them to the chromation step. Tricky business getting consistent staining on sections in any case. You might still be able to use a Cox alternative. I've had good luck with Gibb and Kolb's method on some thick sections although it was described for whole brain (Gibb R, Kolb B. A method for vibratome sectioning of Golgi-Cox stained whole rat brain. J Neurosci Methods. 1998 Jan 31;79(1):1-4.). Good luck, Mike King UF Pharmacology Therapeutics -- Message: 20 Date: Tue, 04 Aug 2009 17:51:17 +0200 From: Dr. Frauke Neff ne...@staff.uni-marburg.de Subject: [Histonet] golgi stain- please help To: histonet@lists.utsouthwestern.edu Message-ID: 20090804175117.z828f2xw3oow8...@home.staff.uni-marburg.de Content-Type: text/plain; charset=ISO-8859-1; DelSp=Yes; format=flowed Dear Histonetters, I was performing a golgi stain on mouse brains and my treated animal group gave nice results but the untreated control group did not show any staining at all (it looks like the staining didn't work). Does anyone know / have an idea, if I could repeat the staining on these vibratome sections? I read something about deimpregnation of golgi stained slides, but found no protocol and I'm not sure if I can perform another fresh golgi stain with these slides. Any suggestion is welcome!!! Thanks to all of you, Frauke ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: reagents for IHC
Are you using automated IHC-staining machinery, Naira? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: WindoPath
Chisti, We've used WindoPath for the last 8 years. Good AP system. Good archives, can make modifications to suit your institution, etc. The only negative that I can give you is: Stay far, FAR away from the 6.0 version. We are using it, but can't wait for the hospital to approve the budget for another upgrade. Our 5.0 version was more stable/user friendly. But we were forced to upgrade as they were no longer supporting 5.0. I can give you further details if needed. Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 3 Date: Mon, 3 Aug 2009 11:39:02 -0700 (PDT) From: Cristi stephenson cls71...@sbcglobal.net Subject: [Histonet] WindoPath To: histonet@lists.utsouthwestern.edu Message-ID: 428592.32791...@web81202.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi all, Does anyone out there currently use WindoPath as their LIS? Any opinions or pointers concerning set-up, usage and the like? Thanks in advance for your guidance, Cristi Stephenson MSA, HT(ASCP) -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RA Lamb paraffin
One of my colleagues has been using paraffin from Raymond A Lamb for embedding rodent brains. She loves it, but now that ThermoFisher has acquired RA Lamb this paraffin is being discontinued. Does anyone know of any other distributors that might still have some stock...or an equivalent substitute paraffin? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laboratory Openings in New York Area
Hello, Below are a few of the positions we are currently working on in NY. We are offering up to $1,000 for referrals if we place qualified candidates in these openings. These positions vary from Histology to Medical Technologist positions. Any help would be appreciated. Ive listed the positions below. nbsp; Special Chemistry Supervisor in Elmwood, NJ. It is a day shift with competitive pay. The candidate must have hplc testing experience. nbsp; Histotech in Rye Brook, Port Chester, New Rochelle, Uniondale, Yonkers, and others in the area. Shifts as well as pay vary as each location is different. Must have Clinical Laboratory Technologist New York licensure. nbsp; Blood Bank Medical Technologist, in West Chester, NY. The candidate must have New York licensure and experience in a blood bank. Also the position is a night shift. nbsp; Blood Bank Supervisor in West Chester, NY. Candidate must have New York Licensure and supervisory experience. The position is a night shift. nbsp; Generalist Lab Manager in Manhattan, NY with a small stat lab. The candidate must have NY Laboratory Supervisor license. They are looking for someone who has supervisory experience with a lab. nbsp; Micro and Hematology Medical Technologist with a reference laboratory in Syosset. The shift is currently not available because it is a new position. The candidate must have their Clinical Laboratory Technologist license in NY. nbsp; Thanks again, and any help is appreciated!!! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 br...@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** nbsp;http://twitter.com/PrometheusBlog nbsp; nbsp; nbsp; ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Several new opportunities with Leica Microsystems
Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. Due to growth we are currently hiring the following positions: Bond Reagents Marketing Manager: - Chicago, IL IHC Sales Specialist - West, Mid-Atlantic, N.TX/OK/AR, and Southeast Field Support Specialist - West Sales Account Executive - West Field Service Engineer - TN Regional Service Manager - Canada Leica offers competitive salary, benefits including medical, dental, vision, prescription, long-term care, life insurance, STD, LTD, and 401 (k). Please forward your resume to: kris.caldw...@leica-microsystems.com Kris Caldwell Human Resources Recruiter Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 www.leica-microsystems.com kris.caldw...@leica-microsystems.com 847-405-5432 - phone 847-236-3035 - fax 847-323-6169- cellular __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Sections
Hi Histonetters, When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. Thanks for your suggestion and help. Peri pnagap...@cau.edu mailto:pnagap...@cau.edu mailto:pnagap...@cau.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Sections
Peri, Sounds like poor infiltration to me. What kind of tissue are we talking about, and what processing program did you use? Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Nagappan, Peri wrote: Hi Histonetters, When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. Thanks for your suggestion and help. Peri pnagap...@cau.edu mailto:pnagap...@cau.edu mailto:pnagap...@cau.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Part-time job opening for an experienced HT or HTL in St. Augustine, Florida. It is a new lab for a group of gastroenterologists. I estimate it will require 4 to 6 hours per day. Very flexible hours; design your own schedule. Pay is commensurate with experience and local scale. Great working conditions. Could be a great second job or an enjoyable first job. Must have 3 to 5 years experience. Probable opening in September 2009. Konni Black Pathology/Lab Development, LLC 253-503-2560 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Advice
Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin Bleeding
We very occasionally have a single slide out of a group of 100 or more where the eosin will bleed after coverslipping. Does anyone know if this is associated with a particular tissue type or treatment with Nair to soften keratin? Thanks, Tresa Veterinary Diagnostic Lab Bozeman, MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hi folks, looking for contact information for a lo...
Hi folks, looking for contact information for a long lost histotech colleague. Looking for a dear friend named Fran Lemons or Fran Walker. Last known to be working in Tennessee. Feel free to contact me with a private e-mail. geowei...@hotmail.com Thank you all, Georgia Weigel, HT(ASCP) 361-960-3886 _ Windows Live™: Keep your life in sync. http://windowslive.com/explore?ocid=PID23384::T:WLMTAGL:ON:WL:en-US:NF_BR_sync:082009 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Sections
You running particularly small specimens like derm? You may need to decrease time in alcohol. Adding a little ammonia water to your ice bath and soaking after facing the block may help too. --Original Message-- From: Kathleen Roberts Sender: histonet-boun...@lists.utsouthwestern.edu To: Nagappan, Peri Cc: Histonet Subject: Re: [Histonet] Paraffin Sections Sent: Aug 4, 2009 1:40 PM Peri, Sounds like poor infiltration to me. What kind of tissue are we talking about, and what processing program did you use? Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Nagappan, Peri wrote: Hi Histonetters, When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. Thanks for your suggestion and help. Peri pnagap...@cau.edu mailto:pnagap...@cau.edu mailto:pnagap...@cau.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor Advice
I recommend the new tissue tech. I use it in my lab and its reliable and intuitive. I strongly warn against TBS. I use one in my other lab and its been nothing but trouble. Its chemical storage is unreliable and leads to cross contamination. --Original Message-- From: Igor Deyneko Sender: histonet-boun...@lists.utsouthwestern.edu To: Histonet Subject: [Histonet] Tissue Processor Advice Sent: Aug 4, 2009 1:43 PM Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] working with mouse liver...
Hi all, We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.) We use commercially made 10% NBF and tried fixing for different length of time with no luck. The processing time is 1 hour in every station. Did anybody working with liver has any suggestions? Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] working with mouse liver...
In my experience you are way over processing the tissue. Drop your processing times to about 30 min per station. That should be fine. Roger Moretz, Ph.D. (ret) - Original Message From: Michelle MacVeigh-Aloni macve...@usc.edu To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, August 4, 2009 7:20:53 PM Subject: [Histonet] working with mouse liver... Hi all, We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.) We use commercially made 10% NBF and tried fixing for different length of time with no luck. The processing time is 1 hour in every station. Did anybody working with liver has any suggestions? Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
