Re: [Histonet] Assay for cell death needed
Dear all, I want to thank you all for sending suggestions to help us finding an appropriate cell death assay. Your comments were really helpful! Alexandra Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.me...@trauma.lbg.ac.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ab Validation
When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ab Validation
We save the slide. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, April 02, 2010 11:19 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ab Validation When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ab Validation
I just save the paperwork. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, April 02, 2010 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ab Validation When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines
Just talked to Jim at CAP and he said the term Processing still can be the transfer of tissue from one container to another (cassette) to be processed in the tissue processor. If there is any analytical thinking involved (dying margins, measuring...) the process becomes Grossing and falls under the high complex rule. Hope that helps. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 booze...@ah.org kim.dona...@bhcpns.org 04/01/2010 09:15 I agree Janice. The only thing it looks to me is to now define Grandfathered in. Because it also states in addition for that criteria as well. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Mahoney,Janice A janice.maho...@alegent.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/31/2010 04:10 PM To 'Joe Nocito' jnoc...@satx.rr.com, Histonet histonet@lists.utsouthwestern.edu cc Subject RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines But above that after the education piece it says in Addition. Jan, Omaha -Original Message- From: Joe Nocito [mailto:jnoc...@satx.rr.com] Sent: Wednesday, March 31, 2010 4:08 PM To: Mahoney,Janice A; Histonet Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the processing and grossing pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe - Original Message - From: Mahoney,Janice A janice.maho...@alegent.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to process tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnelhttp://wwwn.cdc.gov/clia/regs/subpart_m.aspx. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptionshttp://wwwn.cdc.gov/clia/regs/subpart_m.aspx. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your
[Histonet] Cleaning VIP Processor containers
Hello, Does anyone know a good way to clean the inside of the processor solution station containers (we have a Tissue Tek VIP)? Some of our containers have a film on the sides from years of use. We got what we could reach with a scrub brush, but there are some hard to reach areas. Thanks for your help. Brandi Higgins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines
What do you process and don't measure, so everything is grossing. Even our foreskins which are GDO, we take a measurement in our dictation. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, April 02, 2010 11:41 AM To: Janice A Mahoney; kim.dona...@bhcpns.org; histonet-boun...@lists.utsouthwestern.edu Cc: Histonet; 'Joe Nocito' Subject: RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines Just talked to Jim at CAP and he said the term Processing still can be the transfer of tissue from one container to another (cassette) to be processed in the tissue processor. If there is any analytical thinking involved (dying margins, measuring...) the process becomes Grossing and falls under the high complex rule. Hope that helps. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 booze...@ah.org kim.dona...@bhcpns.org 04/01/2010 09:15 I agree Janice. The only thing it looks to me is to now define Grandfathered in. Because it also states in addition for that criteria as well. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Mahoney,Janice A janice.maho...@alegent.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/31/2010 04:10 PM To 'Joe Nocito' jnoc...@satx.rr.com, Histonet histonet@lists.utsouthwestern.edu cc Subject RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines But above that after the education piece it says in Addition. Jan, Omaha -Original Message- From: Joe Nocito [mailto:jnoc...@satx.rr.com] Sent: Wednesday, March 31, 2010 4:08 PM To: Mahoney,Janice A; Histonet Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the processing and grossing pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe - Original Message - From: Mahoney,Janice A janice.maho...@alegent.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to process tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnelhttp://wwwn.cdc.gov/clia/regs/subpart_m.aspx. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptionshttp://wwwn.cdc.gov/clia/regs/subpart_m.aspx. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health
Re: [Histonet] Cleaning VIP Processor containers
dilute acidic acid works wonders on some things. I use vinegar at home for cleaning a multitude of things. Brandi Higgins brandihigg...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/02/2010 09:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Cleaning VIP Processor containers Hello, Does anyone know a good way to clean the inside of the processor solution station containers (we have a Tissue Tek VIP)? Some of our containers have a film on the sides from years of use. We got what we could reach with a scrub brush, but there are some hard to reach areas. Thanks for your help. Brandi Higgins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ab Validation
We save EVERYTHING! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, April 02, 2010 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ab Validation When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New CAP question
ANP 22760: Are new lots of antibody and detection system reagents tested in parallel with old lots? Note: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues. How is everyone out there handling this? What makes up a panel of control tissues? We are currently doing this by staining a control slide and comparing the results to the previous lot. Looking for guidance. Thank you for your help! Stacy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding Beads
Hello out there in Histoland- I am looking for embedding beads, if you happen to know a place to buys them please let me know. Thanks. TGIF September Amspacher HT(ASCP) Technical Specialist - Histology Department Laboratory Chemical Hygiene Officer Bassett Medical Center Cooperstown, New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RCC
If you mean what control do we use for our anti-RCC stain, we use a piece of normal kidney and clear cell renal cell carcinoma. If you were asking about where we purchase the antibody, we get it from Cell Marque. If you wondering about a protocol we use the Ventana Benchmark. Protease 1 for 8 minutes then 8 minutes with anti-RCC, counterstain and bluing. Mark Tarango On Fri, Apr 2, 2010 at 10:13 AM, Howery, Jeffrey jhowe...@yrmc.org wrote: What are people using for a Renal cell Carcinoma. ( this is a test ) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 4bb5bbb8.4aa8.00c...@ah.org
Kathy, What you mentioned to me is completely inconsistent with what I learned when I was in training. ANYONE touching the tissue, especially taking it out of a specimen container and transferring it to a processing cassette, is by definition GROSSING! Gross description (dimensions, color, consistency, friability, etc etc etc) are all included. Every specimen should at least be getting a gross description, even if it isn't processed!!! (Example...foreign body, like a rock or a BB or a stinger from an arthropod, or any foreign object) I seriously question the validity of the quoted CAP standard by this person. Who out there manipulates tissue and doesnt have to describe it? Once again, this I would call a glaring example of the nebulous nature of CAP standards sometimes and the arbitrary interpretations that occur within the organization (and ones like it, depending upon the individual inspector or CAP staffer you talk to. YOU SEEthat is what is really the FACT in all of this discussion. It's all subjectivejust like legal interpretations. So why does CAP get to be in the bully-pulpit, pedantically pontificating to the pathology community as to HOW ITS SUPPOSED to be done? Regards, AB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD5
Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Validation- Keeping track of everything
--- On Fri, 4/2/10, Jeffrey Silverman pathmas...@yahoo.com wrote: From: Jeffrey Silverman pathmas...@yahoo.com Subject: Validation- Keeping track of everything To: laurie.colb...@huntingtonhospital.com Date: Friday, April 2, 2010, 5:34 PM Better stick em in a box labelled with stain date and lot number being validated. Someone's going to want to see them. Here in New York, they are going nuts with new regs. We now have to assign lot numbers to control blocks after we validate them. It doesn't matter that we stick the control on each and every slide we stain and one can plainly determine if the control is adequate or not. If it's not, it has no business being part of the patient record. Now we have to give it a number and track it. Dumb ass stuff if you ask me. In the old days, we'd cut a control slide and if it worked it worked you kept using it and you used it up. If it didn't work, the pathologist would have rejected it and ordered a repeat with a good control. The proof was on each and every slide that it worked. Antibodies and detection chemistries are perishable and can be capricious so validation and inventory tracking are worthwhile IMHO. But fer goodness sake, now we must record the lot numbers of every batch of xylene, alcohol, eosin, etc and track service dates etc. When was the last time anyone had problems with bulk xylene, alcohol, etc. We already do a meticulous daily QC check on the slides, if there's a problem, one can go and check the lot number in use currently, record it and take appropriate action. But, n, the bureaucrats need more to do, so now we must record the lot number of everything that comes in and track the dates that we use it. And they wonder why healthcare is a financial leech bleeding the nation and it's people. I reiterate, sheesh. Happy Springtime everyone. Enjoy the holiday. Jeff Silverman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Bleach works great for the fixative and early alcohol series containers, , dilute 50/50 with water and swish it around, let it sit a while. If it's yellow fatty slime in the later absolute alcohol baths, you must rinse the containers with 50-100 ml of absolute and repeat until a bit of the swill mixed with water doesn't turn white. Oh the fun days of scut work. Jeff ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Good news about grossing
Or is it We measure everything. I think the benchmark should be, if you submit it intact and in its entirety, with no cutting or dissection and no judgement about which or how much to submit, then counting, measuring, weighing, and cassetting biospies or straining or scraping curettings together into a screen counts as processing and requires only basic training and knowlege. But I have the feeling that as long as the pathologist delineates what may be done by whom with what degree of supervision, and regularly evaluates the work of these individuals, that everything will be OK on any inspection. Just be prepared to prove it. Just talked to Jim at CAP and he said the term Processing still can be the transfer of tissue from one container to another (cassette) to be processed in the tissue processor. If there is any analytical thinking involved (dying margins, measuring...) the process becomes Grossing and falls under the high complex rule. Hope that helps. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 booze...@ah.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antibody validation
HI Everyone and Happy Easter! Just wondering how you validate your antibodies? I've read that some antibodies use negative controls with the genes or proteins removed and that the positive controls have the protein added. Is that the procedure for research or manufacturing situations? Thanks, Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Removal of carbon pigment
Hellow all. We have received CT guided FANA smears from lung .The siles are full of carbon particles that mask the under histiocytes and granulomata.W require the removal of carbor particles.Kindly help out to remove them. Muhammad Tahseen Supervisor Histology Javed uz zaman Supervisor Cytology SKMT PAKISTAN LAHORE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
