[Histonet] (no subject)
http://infioratadigerano.org/100298.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vectastain elite ABC kit- multiple dips ok?
Teresa Iglesias wrote: Hi all, I have three 24-well plates with three brain sections in each well. For IHC, do I have to prepare three different/fresh ABC dips for each 24-well screen plate or can I incubate (30min) each successive screen plate into the already used dip of ABC? I would not. Do I have to leave it in longer to allow adequate binding? I may be that the ABC has been consumed by the first one or two 24 well plates? I know you can do this with DAB but you have a visual indicator that you've left it in long enough in that case. Any experience with this? Why not ask Vector? After all, they made the stuff. Geoff Thanks, -Teresa -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: DAB
From: Cynthia Pyse [mailto:cp...@x-celllab.com] Sent: Tuesday, September 28, 2010 3:48 PM To: 'histonet-requ...@lists.utsouthwestern.edu' Subject: DAB Hello Histonetters I need some expert advice concerning the disposal of DAB. Our lab is in NYS and there has been some discussion on the proper way to dispose of DAB. I've done the research, and found household bleach is no longer acceptable. There is the potassium permanganate treatment, the horseradish peroxidase treatment, and also disposing of it the regulated medical waste that is incinerated. What is everyone using? Does NYS have different standards than other states or is this a federal regulation? If you dispose of the solution is the RMW, how do you protect the techs when pouring the waste DAB from our IHC waste container into bottles for disposal? If there is a spill can it be cleaned up within the lab or does a Hazmat team need to be called. I want to make sure we cover all our bases. I don't want any problems with NYS. Thank you in advance for all your input. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problems with listserv
I am having some issues with receiving email from the listserv. I was successfully receiving email and it suddenly stopped. If I have been unsubscribed, please re subscribe my email address. sfe...@cmc-nh.org Thank you, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problems with listserv
* *I think the chatter just died down since many of us are at the NSH symposium. Mark On Wed, Sep 29, 2010 at 8:49 AM, Feher, Stephen sfe...@cmc-nh.org wrote: I am having some issues with receiving email from the listserv. I was successfully receiving email and it suddenly stopped. If I have been unsubscribed, please re subscribe my email address. sfe...@cmc-nh.org Thank you, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problems with listserv
YMMD! You are the first I notice who wants to subscribe, not unscibe or unscribe or unsubscribe :D http://lists.utsouthwestern.edu/mailman/listinfo/histonet I am having some issues with receiving email from the listserv. I was successfully receiving email and it suddenly stopped. If I have been unsubscribed, please re subscribe my email address. sfe...@cmc-nh.org Thank you, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bag Sealing system
Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John /prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt; COLOR: blue This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation. /SPAN/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bag Sealing system
Milestone Medical has a really neat system. www.milesonemed.com It is called the TissueSAFE. You will find it under preanalytical tools. We would love to get one for our couriers and biospecimen repository. We haven't purchased it yet. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masterson_John [masterson_j...@allergan.com] Sent: Wednesday, September 29, 2010 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John /prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt; COLOR: blue This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation. /SPAN/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bag Sealing system
I have been using a food sealer from Walmart (or the likes) for y
ears. You can buy the food seal bags in precut sizes or you can buy
a= roll and make your own. The sealer is around $100 (or less) an= d
the bags vary but are WAY less expansive then buying from a scientific
su= pplier!! The sealers usually last 4-5 years before they don't get
as = hot as they used to (that's if you leave it plugged in all the
time, if you= unplug it each time they last forever almost). Just a
budget saving = idea for you =)
Sarah Goebel, B.A., HT (ASCP)
= /em
Histotechnicia= n
XBiotech USA Inc.
= div8201 East Riverside Dr. B= ldg 4 Suite 100
Austin, Texas 78744
= div
(512)= 386-5107
Original Message
Subject: [Histonet] Bag Sealing system
From: Masterson_John [1]Mas= terson_j...@allergan.com
Date: Wed, September 29, 2010 10:26 am
To: [2]histo...@lists.ut= southwestern.edu
[3]histo...@lists.uts= outhwestern.edu
Hello,
Can anyone recommend a bag and heat sealing system for
archiving/shipping = tissue? Thanks in advance.
John
/prePFONT face=Verdana color=blue size=1= ;SPAN
style=FONT-SIZE: 8pt; COLOR: blue
This e-mail, including any attachments, is meant only for the intended
reci= pient and may be a confidential communication or a communication
privileged= by law. If you received this e-mail in error, any review,
use, disseminat= ion, distribution, or copying of this e-mail is
strictly prohibited. Pleas= e notify the sender immediately of the
error by return e-mail and please de= lete this message from your
system. Thank you in advance for your cooperat= ion.
/SPAN/FONT/P
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References
1. 3Dmailto:masterson_j...@allergan.com;
2. 3Dmailto:histonet@lists.utsouthwestern.edu;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:Histonet@lists.utsouthwestern.edu;
5. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] Bag Sealing system
Cardinal sells the Kapac sealer and a variety of sizes of bags at 2 mm 4 mm bags. This system is industrial and intended for laboratory use. We use to store tissue on-site and transport tissue and cassette between sites. has reduced storage space and incidence of fluid spills. William DeSalvo, B.S., HTL(ASCP) From: masterson_j...@allergan.com To: histonet@lists.utsouthwestern.edu Date: Wed, 29 Sep 2010 10:26:24 -0700 Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John /prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt; COLOR: blue This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation. /SPAN/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] The Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology
HI! I was hoping to find out if I would be able to apply CEU's from the Feb 2011 Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology to the State of Florida Department of Licensing. Does anyone know? There is not a lot of time to get the discounted rate for early registration and I would like to complete the process soon. Thanks for any info, Carrie Disbrow, BS, RVT, HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometry
Hi louise! like Yak-Nam WangI do use Image J. It is very easy to use, you can use just a picture and count particles/cells manually or do it automated after defining the size threshold. The last way It will take you a bit more time until you validate the process, but it is no difficult. cheers, sil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used Microwave processor for sale
We have a used Milestone RHS I Microwave Rapid Histoprocessor that we don't use anymore. We will be remodeling the histology lab in the near future and don't have room for it, so anyone out there in Histoland interested in this piece of equipment? It is in excellent shape and we have lots of extra parts for it. Contact me directly if interested. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Ultraview DAB detection kits for sale.
Hello Histonetters, We are overstocked on Ultraview DAB detection kits and looking to sale some for $2,300.00 per kit. We will pay for shipping within the continental US. The kits are NOT registered with the button still attached. We have 6 with the expiration Date: 4/2011 Lot #812955 We have 6 with the expiration Date: 5/2011 Lot # 812988 Please email me with your contact information if your interested. Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager Amarillo Pathology Associates Amarillo, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Free floating sections
Hello Histonetters! I am currently running a immunofluorescence reaction using free floating brain sections and am having problems with the sections folding as I lift them to mount at the end of the reaction. To obtain the sections, I first perfused the mouse with 4%PFA, dissected the brain and postfixed it in 4%PFA for three hours, washed it twice in PBS solution, and stored the brain in PBS at 4 degrees celcius. Then, I used a vibratome to slice the brain in 100um sections (I have also tried using a cryostat - and tissue tek- but this immuno runs better on sections from the vibratome). After being sliced, the sections were stored at 4 degrees in PBS. I am running the immuno using a culture plate with incubation with a blocker (10%DS in PBS-T), primary antibody incubation overnight at RT, and incubation with secondary antibody at RT for two hours. All these incubations are made under agitation. Also, I am co-staining the cells with the nuclear stain TOPRO. To mount the sections, I am lifting them using a thin paintbrush and placing them into a petri dish filled with PBS. Then I drag the sections on top on a slide, apply Vectashield and the coverslip. Normally the sections should unfold as soon as they are placed in PBS solution, but many of them are staying folded on the brush. I have already tried incubating the primary antibody at 4C and I observed even more folding. Can you suggest anything to prevent this? Thank you very much Alexia Nunez-Parra Graduate Student University of Maryland ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bag Sealing system
Perhaps the best and least expensive tissue save bag sealer I have found can be purchased from ULINE. There are benchtop models that easily fit in a hood, there are floor models. The benchtop model we use cost less than $200 USD, easily fits in a hood, and has been totally trouble free for 2 years now. I hope this helps. Joe Saby, BA HT From: WILLIAM DESALVO wdesalvo@hotmail.com To: masterson_j...@allergan.com; histonet histonet@lists.utsouthwestern.edu Sent: Wed, September 29, 2010 3:25:40 PM Subject: RE: [Histonet] Bag Sealing system Cardinal sells the Kapac sealer and a variety of sizes of bags at 2 mm 4 mm bags. This system is industrial and intended for laboratory use. We use to store tissue on-site and transport tissue and cassette between sites. has reduced storage space and incidence of fluid spills. William DeSalvo, B.S., HTL(ASCP) From: masterson_j...@allergan.com To: histonet@lists.utsouthwestern.edu Date: Wed, 29 Sep 2010 10:26:24 -0700 Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John /prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt; COLOR: blue This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation. /SPAN/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bag Sealing system
Hi Loralee Try a butcher's supply store. They sell devices they use for vacuum packing meat. By removing the air from the bag the evaporation of formalin is almost zero and the specimens will remain intact for many years. The bags are also more hardy than those sold with basic bag sealers. This is also a cheap way of preserving specimens for teaching purposes though depending on the shape of the specimen there can be some distortion due to the vacuum process. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ McMahon, Loralee A loralee_mcma...@urmc.rochester.edu 30/09/2010 3:47 am Milestone Medical has a really neat system. www.milesonemed.com It is called the TissueSAFE. You will find it under preanalytical tools. We would love to get one for our couriers and biospecimen repository. We haven't purchased it yet. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masterson_John [masterson_j...@allergan.com] Sent: Wednesday, September 29, 2010 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John /prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt; COLOR: blue This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation. /SPAN/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello everyone, I have an old Leica RM 2065 microtome that is in need of repair (the clutch stopped working), but according to Leica, the machine is no longer supported by the company. Does anyone have any suggestions for a company or technician who might be able to repair the machine? Thanks in advance, ~Sean ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
